User Manual

30
Table 25. Bead regions for Bio-Plex Pro cytokine assays.
3. Click Format Plate and format the plate according to the plate layout
created in Section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 6).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
blanks
B
, controls
C
, and samples
X
.
Human Cytokines Mouse Cytokines Rat Cytokines
Group I
Bead
Group II
Bead
Group I
Bead
Group II
Bead
Group I
Bead
Region Region Region Region Region
IL-1b 39 IL-1a 63 IL-1a 53 IL-15 42 IL-1a 21
IL-1ra 25 IL-2R
a 13 IL-1b 19 IL-18* 20 IL-1b 28
IL-2 38 IL-3 64 IL-2 36 Basic FGF 25 IL-2 22
IL-4 52 IL-12
p40** 28 IL-3 18 LIF 45 IL-4 33
IL-5 33 IL-16 27 IL-4 39 M-CSF 26 IL-5 52
IL-6 19 IL-18* 42 IL-5 52 MIG 44 IL-6 56
IL-7 74 CTACK 72 IL-6 38 MIP-2 27 IL-7 38
IL-8 54 GRO-
a 61 IL-9 33 PDGF-BB 35 IL-10 19
IL-9 77 HGF 62 IL-10 56 VEGF 47 IL-12
p40** 76
IL-10 56 IFN-
a2 20 IL-12p40 76 IL-12p70 78
IL-12
p70 75 LIF 29 IL-12p70 78
Group III
IL-13 15
IL-13 51 MCP-3 26 IL-13 37 IL-17A 72
IL-15 73 M-CSF 67 IL-17 72 CD40L 30 IL-18* 20
IL-17 76 MIF 35 Eotaxin 74 IL-17F 28 EPO 14
Eotaxin 43 MIG 14 G-CSF 54 IL-21 14 G-CSF 54
Basic FGF 44
b-NGF 46 GM-CSF 73 IL-22 15 GM-CSF 37
G-CSF 57 SCF 65 IFN-
g 34 IL-23p19 61 GRO/KC 57
GM-CSF 34 SCGF-
b 78 KC 57 IL-31 29 IFN-g 34
IFN-y 21 SDF-1
a 22 MCP-1 (MCAF) 51 IL-33 13 M-CSF 26
IP-10 48 TNF-
b 30 MIP-1a 77 MIP-3a 12 MCP-1 51
MCP-1 53 TRAIL 66 MIP-1
b 75 MIP-1a 77
MIP-1
a 55
Group II
RANTES 55
Group III
MIP-2*** 27
MIP-1
b 18
Singleplexes
TNF-a 21
Singleplexes
MIP-3a 36
PDGF-BB 47 RANTES 55
RANTES 37 ICAM-1 12 ICAM-1 22 TNF-
a 43
TNF-
a 36 V-CAM1 15 IL-25 (IL-17E) 67 VEGF 47
VEGF 45 IL-27
p28 43
* IL-18 is not available as a singleplex assay but only as part of an assay panel.
** IL-12p40 cannot be multiplexed with IL-12p70.
*** MIP-2 cannot be multiplexed with GRO/KC.