User Manual

12
Assay Low RP1 (PMT) High RP1 (PMT)
Human cytokines (group l, ll)
•
User validation required*
Mouse cytokines (group l, ll, III)
•
User validation required*
Rat cytokines (group l) User validation required*
•
Low RP1 (PMT), broad High RP1 (PMT), narrow
Cross-panel mixing
range cytokine curve range cytokine curve
Human cytokines + diabetes
•
Mouse cytokines + diabetes User validation required*
•
Rat cytokines + diabetes
•
RP1 (PMT) Setting for Standard Curves
The Bio-Plex 200 and 3D systems have two RP1 (PMT or photomultiplier
tube) setting options, while the Bio-Plex
®
MAGPIX
™
has no PMT and
therefore no PMT setting options. Instead, MAGPIX uses default instrument
settings similar to low PMT on the Bio-Plex 200 (Table 6).
Table 6. Overview of PMT setting options on Bio-Plex systems.
The Bio-Plex Pro
™
human and mouse cytokine assays were developed
on the low PMT setting using the Bio-Plex 200 system, while the rat
cytokine assays were developed on the high PMT setting. Protocols using
alternative standard dilution series or different PMT settings should be
validated by the end user, for example when mixing the cytokine assays
with diabetes assays (Table 7 and Table 24).
Table 7. Settings for optimal sensitivity on the Bio-Plex 200 system*.
* Contact Bio-Rad technical support for the most up to date recommendations on PMT
settings and cross-panel multiplexing compatibility.
Reconstitute a Single Vial of Standards
This procedure prepares enough material to run each dilution in duplicate.
1. Gently tap the vial containing the lyophilized standard.
Instrument RP1 (PMT)
Bio-Plex 100, 200* Low, high
Bio-Plex 3D* Standard, enhanced
Bio-Plex MAGPIX* N/A, use default instrument settings
* Or similar Luminex-based system.