User Manual

11
Bio-Rad Cell Lysate Controls
The positive and negative cell lysate controls are used for qualitative
verification of assay performance. Refer to Table 3 to select the
appropriate controls for your phosphoprotein or total target of interest.
Table 3. Selection guide for Bio-Rad cell lysate controls.
Phosphoprotein of Interest Lysate Control Catalog #
Akt (Ser
473
) GSK-3a/b (Ser
21
/Ser
9
)
Akt (Thr
308
) MEK1 (Ser
217
/Ser
221
) EGF-treated HEK-293 171-YZ0001
Erk1/2 (Thr
202
/Tyr
204
, Thr
185
/Tyr
187
)
ATF-2 (Thr
71
) JNK (Thr
183
/Tyr
185
)
c-Jun (Ser
63
) p38 MAPK (Thr
180
/Tyr
182
) UV-treated HEK-293 171-YZ0009
CREB (Ser
133
) p53 (Ser
15
)
Btk (Tyr
223
) Pl3K p85 (Tyr
458
)
Lyn (Tyr
507
) Syk (Tyr
352
)
H
2
O
2
-treated Ramos 171-YZ0011
c-AbI (Tyr
245
) Untreated K-562 171-YZT003
EGFR (Tyr
1068
) EGFR (Tyr
1173
) EGF-treated HeLa 171-YZ0002
HER-2 (Tyr
1248
) p90 RSK (Ser
380
)
HSP27 (Ser
78
) S6 ribosomal protein EGF-treated SK-BR-3 171-YZ0003
(Ser
235
/Ser
236
)
IGF-1R (Tyr
1131
) IR-b (Tyr
1146
) IGF-1-treated HEK-293 171-YZ0005
IkB-a (Ser
32
/Ser
36
) Smad2 (Ser
465
/Ser
467
)
NF-kB p65 (Ser
536
)
TNF-a–treated HeLa 171-YZ0008
p70 S6 Kinase (Thr
421
/Ser
424
) p70 S6 Kinase (Thr
389
) NGF-b–treated PC12 171-YZ0006
BAD (Ser
136
) PDGFR-a (Tyr
754
)
IRS-1 (Ser
636
/Ser
639
) PDGFR-b (Tyr
751
) PDGF-treated NIH3T3 171-YZ0007
mTOR (Ser
2448
) PTEN (Ser
380
)
Stat1 (Tyr
701
) Stat3 (Tyr
705
)
Stat3 (Ser
727
)
IFNa-treated HeLa 171-YZ0004
Src (Tyr
416
) Src-transfected NIH3T3 171-YZ0013
VEGFR-2 (Tyr
1175
) VEGF-treated HUVEC 171-YZ0010
ZAP70 (Tyr
319
) H
2
O
2
-treated Jurkat 171-YZ0012
Negative control for all phosphoprotein assays Phosphatase-treated HeLa 171-YZB001
continues