Instruction Manual

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b. Click the Add button. Enter the bead region number and name
for the first analyte. Click Add Continue to repeat for each
analyte in the assay.
For reference, bead regions are shown in Table 10.
c. Click the Add button when the last analyte has been added and
click OK to save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click the Add All button.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking on Rename Panel and entering a
new panel name.
3. Click Format Plate and format according to the plate template
created in section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
blanks
B
, controls
C
, and samples
X
. Note that
Bio-Plex Manager automatically subtracts the blank FI
value from all other assay wells.
Analyte Bead Region Analyte Bead Region
lgG
1
54 lgA 12
lgG
2
21 lgM 56
lgG
3
36 lgE 38
lgG
4
72 lgG Total 66
Table 10. Bead regions for Bio-Plex Pro isotyping assays.