User guide

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Appendix: Non-Human Primate (NHP)
Cross-Reactivity
The human chemokine assays were found to be cross-reactive with
two common NHP species, cynomolgus (Cyno) macaque and rhesus
macaque. Other NHP species were not tested. The degree of cross-
reactivity was determined based on the ability of each assay to detect
native analyte in serum, plasma and/or culture media from mitogen
stimulated peripheral blood mononuclear cells (PBMC).
Assay signal (MFI) for the most highly cross-reactive samples were compared
against the human standard curve and scored as shown in the Table 14.
Table 14. NHP cross-reactivity of the human chemokine assays.
Analyte Cyno Rhesus Analyte Cyno Rhesus
6Ckine/CCL21 – – IL-8
+++ +++
BCA-1/CXCL13
+++ +++
IP-10/CXCL10
+++ +++
CTACK/CCL27 – – I-TAC/CXCL11
+++ +++
ENA-78/CXCL5
+ +
MCP-1/CCL2
+++ +++
Eotaxin/CCL11
+ +
MCP-2/CCL8
++ ++
Eotaxin-2/CCL24 – – MCP-3/CCL7 – –
Eotaxin-3/CCL-26 – – MCP-4/CCL13
+++ +++
Fractalkine/CX3CL1
+++ +
MDC/CCL22
+
–
GCP-2/CXCL6 – – MIF
+++ +++
GM-CSF
+++ +++
MIG/CXCL9 – –
Gro-a/CXCL1 – – MIP-1a/CCL3
+++ +++
Gro-b/CXCL2
+ +
MIP-1δ/CCL15 –
+
I-309/CCL1 – – MIP-3a/CCL20 – –
IFN-g – – MIP-3b/CCL19
++ ++
IL-10
+++ +++
MPIF-1/CCL23 – –
IL-16
+ +
SCYB16/CXCL16 –
+
IL-1b
+++ +++
SDF-1a+b/CXCL12
+ +
IL-2 – – TARC/CCL17 – –
IL-4
++ +
TECK/CCL25
+ +
IL-6
+++ +++
TNF-a
+
–
– No NHP cross-reactivity observed. Assay signal below S6 on the standard curve.
+
Some NHP cross-reactivity observed. Assay signal above S6.
++
Moderate NHP cross-reactivity observed. Assay signal above S5.
+++
Strong NHP cross-reactivity observed. Assay signal above S3.