User guide

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a. Click Add Panel
in the Select Analytes toolbar. Enter a new
panel name. Select Bio-Plex Pro Assay Magnetic from the
assay dropdown list. If using Bio-Plex Manager version 5.0 or lower,
select MagPlex from the assay dropdown list.
b. Click Add. Enter the bead region number and name for the first
analyte. Click Add Continue to repeat for each analyte in the
assay. Refer to the bead regions in parentheses ( ) listed on the
peel-off label provided with the standards.
For reference, bead regions are shown in Table 13.
c. Click Add when the last analyte has been added and click OK to
save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click Add All.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking Rename Panel and entering a new
panel name.
Table 13. Bead regions for the human chemokine panel.
Analyte Bead Region Analyte Bead Region Analyte Bead Region
6Ckine/CCL21 12 IL-1b 39 MDC/CCL22 29
BCA-1/CXCL13 74 IL-2 38 MIF 35
CTACK/CCL27 72 IL-4 52 MIG/CXCL9 14
ENA-78/CXCL5 73 IL-6 19 MIP-1a/CCL3 55
Eotaxin/CCL11 43 IL-8/CXCL8 54 MIP-1δ/CCL15 66
Eotaxin-2/CCL24 30 IL-10 56 MIP-3a/CCL20 62
Eotaxin-3/CCL26 65 IL-16 27 MIP-3b/CCL19 76
Fractalkine/CX3CL1 77 IP-10/CXCL10 48 MPIF-1/CCL23 37
GCP-2/CXCL6 15 I-TAC/CXCL11 25 SCYB16/CXCL16 64
GM-CSF 34 MCP-1/CCL2 53 SDF-1a+b/CXCL12 22
Gro-a/CXCL1 61 MCP-2/CCL8 57 TARC/CCL17 67
Gro-b/CXCL2 78 MCP-3/CCL7 26 TECK/CCL25 46
I-309/CCL1 20 MCP-4/CCL13 28 TNF-a 36
IFN-g 21