User guide

ManualsBrandsBio-Rad ManualsAccessories for waterBio-Plex Pro™ Human Chemokine Assays

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Table of Contents

Introduction 1

Principle 2

Kit Contents and Storage 4

Recommended Materials 5

Assay Workﬂow 6

lmportant Considerations 7

Detailed Instructions 7

1. Plan Plate Layout 8

2. Prepare Instrument 9

3. Prepare Wash Method 10

4. Prepare Wash Buffer 11

5. Prepare Standards and Controls 11

6. Prepare Samples 14

7. Prepare Coupled Beads 16

8. Run Assay 18

9. Read Plate 22

Troubleshooting Guide 29

Appendix: Non-Human Primate (NHP) Cross-Reactivity 34

Plate Layout Template 35

Calculation Worksheet 36

Safety Considerations 38

Legal Notices 38

Ordering Information 39