Manual

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7. Select Step 5 (Enter Controls Info) to enter controls information. This
is where the concentration of the user-specified controls is entered
into the protocol.
a) If necessary, deselect the box for same concentration values for
all analytes.
b) Select an analyte from the pull-down cell.
c) Enter the description and dilution information for each user-
specified control.
d) Repeat steps 7b and 7c for each additional analyte in the assay.
8. Select Step 6 (Enter Sample Info) and enter sample information.
Remember to enter the appropriate dilution factor information.
9. Select Step 7 (Run Protocol). Then:
a) Choose 100 beads per region.
b) In Advanced Settings, set the bead map to 25 region.
c) In Advanced Settings, set the sample size to 50 µl.
d) In Advanced Settings, confirm that the DD gate is set to 5,000
(low) and 32,000 (high).
e) Save the protocol (.pbx) file.