Bio-Plex Manager 4.1 TM Software Installation lnstructions For workstations with IS 2.
Table of Contents Section 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Section 2 Upgrade Requirements . . . . . . . . . . . . . . . . . . . . 2 Instrumentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Materials Provided . . . . . . . . . . . . . . . . . . . . . . . . . 3 Section 3 Software Installation . . . . . . . . . . . . . . . . . . . . . . .4 Install Bio-Plex Manager 4.1 Software . . . . . . . . . . 4 Section 4 Firmware Verification . . . . . . .
Section 1 Introduction These instructions are part of a kit specifically developed to enable using Bio-Plex Manager version 4.1 software on workstations with IS 2.3 software. Details are provided for installing Bio-Plex Manager 4.1 software and verifying the firmware located on each of the array reader (analyzer), microplate platform (XY platform), and HTF (sheath delivery system). These instructions should be followed precisely as written. Once Bio-Plex Manager 4.
Section 2 Requirements Instrumentation Identify the serial numbers of each of the array reader, microplate platform, and HTF in order to determine whether they meet the minimum requirements.
Section 3 Software Installation Prior to proceeding with the software installation, ensure that the minimum requirements for the array reader, microplate platform and HTF have been met (refer to Section 2 – Requirements). It is important to follow the installation instructions precisely as written. If problems are encountered, contact your local Bio-Rad office, or in the US, call 1-800-4BIORAD (1-800-424-6723). Install Bio-Plex Manager 4.1 Software 1. Insert the Bio-Plex Manager 4.
Section 4 Firmware Verification 3. Prior to using the newly installed Bio-Plex Manager 4.1 software, verify the firmware located on each of the array reader, microplate platform, and HTF as follows. Both the Detector Device box (for the array reader) and the Movement Device box (for the microplate platform) will show that the program is detecting the devices (the Movement Device for the SD Device (HTF) will be addressed later).
5. If the message in the Detector Device box states that the LX100 firmware version is already 2.3.9, skip to Verify Mircoplate Platform Firmware below. However, if the LX100 firmware version is not 2.3.9 and the message indicates that the upgrade to version 2.3.9 is available, select Upgrade and then Yes to download version 2.3.9 firmware to the array reader. It may take a few minutes to download the firmware and initialize the instrument. Select OK when the download is complete.
Verify HTF Firmware If the system includes an HTF, continue following these instructions. If the system does not include an HTF, the firmware verification is complete (skip to Reset Instrument Pressure Settings in Section 5). 1. 2. 3. 10 4. If the HTF has a cable connecting it to the array reader, disconnect this cable at the HTF. Then disconnect the serial cable from the microplate platform and connect it to the HTF. The serial cable should now connect the HTF to the computer. Power on the HTF.
Section 5 Pressure Setting Adjustment Verify HTF Pressure Settings 1. Launch Bio-Plex Manager 4.1 software. From the menu bar, select Instrument | Additional Functions | Prime. Select OK to begin priming the array reader and HTF. 2. 7. Run prime once more to verify the pressure is set correctly by observing that it stabilizes at the correct pressure (+0.2 psi). Reset Instrument Pressure Settings 1. Close the Bio-Plex Manager 4.1 software application. Select Start | All Programs | Bio-Plex Manager 4.
6. Exit the application and power off the array reader. Then power on the array reader and restart the Bio-Plex Manager 4.1 software application. The following two dialog boxes will appear in succession: 7. To restart the array reader, first exit from Bio-Plex Manager. Cycle the power on the array reader and relaunch Bio-Plex Manager. Congratulations—the Bio-Plex Manager 4.
