Bio-Plex Manager™ MP Software Getting Started Guide Version 1.
Bio-Plex Manager™ MP Software Getting Started Guide Version 1.
Bio-Rad Technical Support Department The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Time. Worldwide technical support is available on the Web at www.consult.bio-rad.com. Phone: 1-800-424-6723, option 2 Fax: 1-510-741-5802 Email: LSG.TechServ.US@Bio-Rad.com (U.S.) LSG.TechServ.Intl@Bio-Rad.com (International) Web: www.consult.bio-rad.
Table of Contents Chapter 1 Installing and Preparing Your System . . . . . . . . . . . . . . . . .5 Required Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Setting up your MAGPIX Instrument and Computer. . . . . . . . . . . . . . . . . . . . . . . . 6 Differences Between Bio-Plex Manager MP and xPONENT . . . . . . . . . . . . . . . . . 7 Next Steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Table of Contents Appendix A License Activation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Activating the Bio-Plex Manager MP License . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Activating the License Online . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Activating the License through Technical Support . . . . . . . . . . . . . . . . . . . . . . . . 39 Generating the Credentials File . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1 Installing and Preparing Your System This chapter gives you an overview of how to get your MAGPIX instrument and your computer installed and prepared for use. Required Materials You will need the following materials to complete setting up the MAGPIX instrument.
1 | Installing and Preparing Your System Setting up your MAGPIX Instrument and Computer To set up your MAGPIX instrument and computer 1. Follow the instructions for your situation: You just purchased the MAGPIX instrument from Bio-Rad. Follow the instructions in the Computer and MAGPIX Assembly Instructions (part #10020326) that are included in the MAGPIX shipping crate. You were using the xPONENT software to run the MAGPIX instrument and you now want to use the Bio-Plex Manager MP software.
Differences Between Bio-Plex Manager MP and xPONENT Differences Between Bio-Plex Manager MP and xPONENT If you have used xPONENT software with MAGPIX, there are some differences in how Bio-Plex Manager MP software works that you should be aware of: Adjusting probe height — adjusting the probe height is simple and reproducible. The software uses the probe height adjustment plate to set the probe height for the two locations in the off-plate reagent area and four heights for consumables.
1 | Installing and Preparing Your System 8 Bio-Plex Manager MP Software |
2 An Overview of Bio-Plex Manager MP Bio-Plex Manager™ MP Quick Guide 1 Run Daily Start Up at the start of each day. 1. Navigate to the Maintenance view. 2. Follow the instructions in the application window. 3. Click Start. 2 Create and run your protocols. 1. Navigate to the Create/Run Protocols view. 2. Click New in the toolbar. 3. Click Run in the toolbar to run the protocol 4. Save the protocol to reuse it later. 3 Export and analyze your results. Export your results. Use Bio-Plex Manager 6.
2 | An Overview of Bio-Plex Manager MP Run the Daily Start Up Routine To run the Daily Start Up routine 1. Start Bio-Plex Manager MP software. Bio-Plex Manager MP opens to the Maintenance view. From the Maintenance view, you run the routines required to keep the MAGPIX instrument in good working order. Bio-Plex Manager MP keeps track of the status of the instrument so you do not have to remember which routines need to be run each day. 2.
Run the Daily Start Up Routine In the Maintenance view, the Reagent Block section provides information on filling the reservoirs and wells with the appropriate liquids and reagents. Click the Description arrow to display or hide a brief explanation of the routine.
2 | An Overview of Bio-Plex Manager MP After the routine has been successfully run, None is displayed indicating that maintenance of the instrument is up to date. Note: There may be times when you have to run more than one recommended routine before maintenance is up to date. For example, Enhanced Maintenance may be displayed as the recommended routine if calibration or verification fails.
Create the Protocol Create the Protocol To create the protocol 1. Click Create/Run Protocols in the navigation bar to open the Create/Run Protocols view. From this view, you create and run your protocols.
2 | An Overview of Bio-Plex Manager MP 2. Click New in the toolbar to open the protocol dialog box. You create your protocols in the protocol dialog box. You click each of the buttons in the navigation bar to select the analytes, format the plate, and define the standards used.
Create the Protocol 3. Click Select Analytes in the protocol dialog box to choose your assay panel and select your analytes. You select the assay panel from the Assay Panel dropdown list. Once you make your selection, the pane is populated with a list of the analytes in the panel.
2 | An Overview of Bio-Plex Manager MP Select the analytes you want displayed in your results. The assay you purchased may include only a subset of the possible analytes. Refer to the product literature for a list of the included analytes. There may be times when your assay includes analytes from two or more assay panels. You can combine analytes from multiple assay panels using the Manage Assay Panels feature.
Create the Protocol 4. Click Format Plate to specify the types of wells in your plate. Click the button in the toolbar to select the type of sample, then click the squares in the template to format your wells with that sample type. Blank Control Standard Unknown Autofill buttons Format Plate You can easily create replicate groups or format multiple wells at one time using the Autofill buttons. For more information on these features, see the online help for the software.
2 | An Overview of Bio-Plex Manager MP 5. Click Standards Info to choose the standard lot you are using with your experiment. You select the standard lot you are using with your panel from the Standard Lot list and specify the dilution factor. Note: You will not be able to specify the standards information until you select your analytes and format your plate with standard wells.
Create the Protocol If the standards information is not available for your standard lot or you want to use custom values, then you must create a new standard lot. You do this with the New Standard Lot button. New Standard Lot Manage Standard Lots You have completed your protocol definition and you are now ready to run the protocol.
2 | An Overview of Bio-Plex Manager MP Run the Protocol To run the protocol 1. Click Create/Run Protocols to go to the Create/Run Protocols view. 2. Click Start in the toolbar to open the Run Settings dialog box.
Run the Protocol 3. Specify the settings for your run in the Run Settings dialog box. Select the type of plate you are using in the Plate Type field. Refer to the table below for the description of the supported plate types. 4.
2 | An Overview of Bio-Plex Manager MP Bio-Plex Manager MP populates the Create/Run Protocols view with the results.
Run the Protocol If there are errors in your experiment, the wells with the error display a warning symbol and the table displays a warning symbol for the analytes. Click the Alerts icon to display information about the error. If a warning icon appears in the status bar, then maintenance is out of date.
2 | An Overview of Bio-Plex Manager MP Analyze Your Data in Bio-Plex Manager 6.x You create and run your protocols in Bio-Plex Manager MP and you analyze your data in Bio-Plex Manager 6.x. To analyze your data you first export the result to a file. Note: Analysis of Bio-Plex Manager MP results is supported only with Bio-Plex Manager 6.0 or higher. Earlier versions of Bio-Plex Manager are not supported. To analyze your data 1.
Analyze Your Data in Bio-Plex Manager 6.x 2. Follow the prompts to save your results to a file. After the file is saved, Bio-Plex Manager MP prompts you to open Bio-Plex Manager 6.x to analyze your results. (You will see this prompt only if Bio-Plex Manager 6.x is installed on your computer.) For more information on analyzing your results, see Chapter 3, Analyzing Your Data.
2 | An Overview of Bio-Plex Manager MP Run the Shut Down Routine When you are done for the day, return to the Maintenance view and run the Shut Down routine. Shut Down routine The Shut Down routine cleans the fluidics lines and prevents the buildup of debris within the system. Bio-Rad recommends you run this routine each day you use the MAGPIX system to maintain your instrument.
3 Analyzing Your Data You must first export your data to a file. Then this data file can be analyzed in Bio-Plex Manager™ 6.x or other analysis software. Exporting Your Data from the Analyze/Export View You can analyze your data immediately after running your protocol using the Export Results command as described in this chapter. Or you can return at a later time and export your results from the Analyze/Export view into Bio-Plex Manager 6.x. To export your result from the Analyze/Export view 1.
3 | Analyzing Your Data 2. Select the result you want to export and click Export Results. 3. Follow the prompts to save your results to a file. 4. Bio-Plex Manager MP prompts you to open Bio-Plex Manager 6.1 (or whatever version of the software is installed on your machine) to analyze your results. Click Yes. Bio-Plex Manager 6.x displays the results.
Optimizing the Standard Curve in Bio-Plex Manager 6.x Optimizing the Standard Curve in Bio-Plex Manager 6.x After you open your results in Bio-Plex Manager 6.x, follow these steps to optimize your results. To optimize the standard curve 1. Click Standard Curve in the navigation bar.
3 | Analyzing Your Data 2. Select “Apply across all analytes” and click Optimize.
Optimizing the Standard Curve in Bio-Plex Manager 6.x 3. Review the optimization report and identify any analytes for which there are comments or that have not been successfully optimized. It will be difficult to generate meaningful results if many of your unknowns do not fall within the usable range of the curve, that is, between the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).
3 | Analyzing Your Data Analyzing Your Data Using Other Software You can export your data to other applications to group biological replicates for further analysis. To analyze your data 1. Click Report Table in the navigation bar. 2. Click “Export results to Bio-Plex Data Pro” to view your results in Bio-Plex Data Pro™, or click Export Report Table to view your results in Microsoft Excel.
4 Maintaining the MAGPIX Instrument This chapter describes the periodic maintenance that must be performed on the MAGPIX instrument to keep it working according to the manufacturer’s specifications. Daily Maintenance Each day you use the MAGPIX instrument, perform these maintenance tasks: At the beginning of the day — at the beginning of each day you use the instrument, run the recommended routine. Check the Recommended Routine section in the Maintenance view.
4 | Maintaining the MAGPIX Instrument Cleaning the Sample Probe To clean the sample probe 1. Turn off the MAGPIX instrument and unplug the power cord. 2. Remove the sample probe. 3. Use either or both methods to clean the sample probe: Bath sonicator — place the tip of the sample probe in the bath sonicator for 2–5 min. 10 ml syringe — force distilled water through the tip of the sample probe to its large end. 4. Replace the sample probe.
MAGPIX Maintenance MAGPIX Maintenance Within the first year of use, Bio-Rad recommends that you perform the 6-month and 12-month maintenance. The maintenance kits are: Bio-Plex® MAGPIX™ 6-Month Preventive Maintenance Kit — cleans the air filters and replaces the syringe seals (catalog #171-012010). Bio-Plex MAGPIX 12-Month Preventive Maintenance Kit — replaces the sample probe tube and the drive fluid filter (catalog #171-012012).
4 | 36 Maintaining the MAGPIX Instrument | Bio-Plex Manager MP Software
A License Activation To have access to all of the features of Bio-Plex Manager™ MP software, you must activate the product with a valid product license. You will need a separate license for each computer on which you install Bio-Plex Manager MP. Note: If you purchased a new MAGPIX instrument, the computer included with the instrument comes with the software already activated. Therefore, you can skip this step.
A | License Activation 2. If the product license has not been activated, the Product Activation Required window appears. 3. Select Activate Bio-Plex Manager MP Online and click Continue. 4. Enter your activation code in the text box and click Activate. Note: The activation code can be found in the CD folder.
Activating the License through Technical Support The Activation Successful window appears confirming that the software has been activated. 5. Click OK to exit the activation software and start Bio-Plex Manager MP. Activating the License through Technical Support If the computer on which Bio-Plex Manager MP is installed does not have an Internet connection, you can request a product license from Bio-Rad Technical Support to activate the software. This is a two-phase process: 1.
A | License Activation Generating the Credentials File To generate the credentials file 40 1. Start the Bio-Plex Manager MP software. 2. If the product license has not been activated, the Product Activation Required window appears. 3. Select Activate Bio-Plex Manager MP through Technical Support and click Continue.
Activating the License through Technical Support 4. Select Send credentials to Technical Support and click Continue. 5. Enter your activation code in the text box and click Save. Note: The activation code can be found in the CD folder. 6. In the Save Licensing Credentials to a File dialog box, enter the name of the file and click Save.
A | License Activation The File Successfully Saved window appears with instructions on how to send the credentials file to Technical Support. 7. Move the credentials file to a computer where you can send email, and email the file to Bio-Rad Technical Support (LSG_Software_Act@bio-rad.com). Activating the Software with a License File Technical Support will generate a file with the license and email the file to you.
Activating the License through Technical Support To activate Bio-Plex Manager MP with the license file 1. Start the Bio-Plex Manager MP software. 2. In the Product Activation Required window, select Activate Bio-Plex Manager through Technical Support and click Continue. 3. Select “Import license file and activate product” and click Continue.
A | License Activation 4. Click Activate. 5. Navigate to where the license file is located, select the file, and click Open. The Activation Successful window appears confirming that the software has been activated. 6. 44 Click OK to exit the activation software and start Bio-Plex Manager MP.
Bio-Rad Laboratories, Inc. Web site www.bio-rad.
