User Manual

Table of Contents
Page
Section 1 Introduction ...................................................................................1
1.1 Specifications ............................................................................................1
Section 2 Special Handling Features............................................................2
2.1 Autoclaving ................................................................................................2
2.2 Chemical Stability ......................................................................................2
Section 3 Bio-Dot Assembly .........................................................................3
3.1 Assembly...................................................................................................3
3.2 Helpful Hints ..............................................................................................5
Section 4 Protein Blotting .............................................................................7
4.1 Immunoassay Procedure ...........................................................................7
4.2 Special Protein Blot Applications................................................................9
Section 5 DNA Blotting................................................................................10
Section 6 RNA Blotting ................................................................................11
6.1 Alkaline RNA Denaturation and Fixation ..................................................11
6.2 Glyoxal RNA Denaturation and Fixation...................................................11
Section 7 Hybridization Protocols for Nucleic Acids ................................13
7.1 Probe Recommendations ........................................................................13
7.2 Hybridization Protocols for DNA or RNA Bound to Nitrocellulose
or Zeta-Probe
®
Membrane.......................................................................13
7.3 Hybridization Protocols for RNA Probes...................................................15
7.4 Probe Stripping and Rehybridization ........................................................15
Section 8 Solutions for Protein Applications.............................................17
8.1 Solutions for Nitrocellulose Membrane.....................................................17
8.2 Solutions for Zeta-Probe Membrane for Protein Applications ...................18
Section 9 Solutions for Nucleic Acid Applications....................................19
Section 10 Troubleshooting Guide ...............................................................21
Section 11 Applications and References .....................................................24
12.1 Common Applications..............................................................................24
12.2 References ..............................................................................................22
Section 12 Ordering Information ..................................................................27