User Manual

Section 11
Applications and References
12.1 Common Applications
Protein
Radioimmunoassay (RIA), enzyme-linked immunoassay (EIA), fluoroimmunoassay (FIA) of
soluble or particulate cellular antigens (Cleveland et al. 1981, Shen et al. 1980)
Analysis of enzymes (Faulstich et al. 1974, Huet et al. 1982)
Hormone-receptor assays (Gershoni and Palade 1983, Schafer et al. 1974)
Immunoglobulin detection (Herbrink et al. 1982, Wang et al. 1980)
Hybridoma screening (Bennett and Yeoman 1983, Hawkes et al. 1982, Horejsi and Hilgert
1983, Kane et al. 1982, Locker and Motta 1983, Shen et al. 1980)
DNA-binding proteins (Achberger and Whiteley 1981, Allen and Parsons 1979, Karagyozov
and Hadjiolov 1982, Lin and Riggs 1975, Lye and Birge 1981)
Glycoprotein, lectin assays (Gershoni and Palade 1983, Neuhoff et al. 1981)
Viral antigen analysis, clinical applications (Cleveland et al. 1981, Richman et al. 1981,
1982)
Column or gel-column fraction monitoring (Cunningham 1983, Palfree and Elliott 1982,
Shen et al. 1980)
Antibody purification (Olmsted 1981)
Total protein microassay (Nakamura et al. 1985)
Nucleic Acid
Dot hybridization of DNA/RNA for sequence homology, sequence abundance, etc. (Kaftos
et al. 1979, Thomas 1980)
Antibody detection of DNA (Tron et al. 1983)
Viral DNA sequence detection (Berg et al. 1986, Brandsma and Miller 1980)
Hybridization selection (Harpold et al. 1978, Ricciardi et al. 1979)
Gene product and clone selection (Kranz and Gennis 1982)
Plasmid analysis (Bresser and Gillespie 1983)
CsCl, gel-column, or sucrose-gradient fraction (Cunningham 1983, Palfree and Elliott 1982,
Shen et al. 1980)
DNA filter-binding assays, including DNA-drug, DNA-protein, virus-host, DNA synthesis,
etc. (Bresser and Gillespie 1983)
DNA/RNA purification (Chen and Thomas 1980, Holland and Wangh 1983, Kutateladze et
al. 1979, Winberg and Hammarskjold 1980)
12.2 References
Achberger EC and Whiteley HR, The role of the delta peptide of the Bacillus subtilis RNA polymerase
in promoter selection, J Biol Chem 256, 7424–7432 (1981)
Allen JD and Parsons SM, Nitrocellulose filter binding: quantitation of the histidyl-tRNA-ATP
phosphoribosyltransferase complex, Anal Biochem 92, 22–30 (1979)
Bennett FC and Yeoman LC, An improved procedure for the 'dot immunobinding' analysis of
hybridoma supernatants, J Immunol Methods 61, 201–207 (1983)
Berg LJ et al., Complementation of a bovine papilloma virus low-copy-number mutant: evidence for a
temporal requirement of the complementing gene, Mol Cell Biol 6, 859–869 (1986)
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