User Manual

Section 9
Solutions for Nucleic Acid Applications
20x SSC
3 M NaCl
0.3 M trisodium citrate (FW = 294.1)
Dissolve 175.0 g NaCl and 88.2 g trisodium citrate in ddH
2
O. Adjust volume to 1 L with
ddH
2
O.
20x SSPE
3.6 M NaCl
0.2 M Na
2
HPO
4
•7H
2
O
0.02 M EDTA
Dissolve 210.0 g NaCl, 53.6 g Na
2
HPO
4
•7H
2
O, 7.44 g EDTA ddH
2
O. Adjust volume to 1 L
with ddH
2
O.
TE
10 mM Tris-HCl, pH 8.0
1 mM EDTA, pH 8.0
Dilute 10 ml 1 M Tris-HCl, pH 8.0 and 4 ml 0.250 M EDTA, pH 8.0 to 1 L with ddH
2
O.
100x Denhardt’s Solution
2% bovine serum albumin
2% polyvinylpyrrolidone
2% Ficoll
Dissolve 2.0 g BSA, 2.0 g polyvinylpyrrolidone, 2.0 g Ficoll in ddH
2
O. Adjust volume to 100
ml with ddH
2
O.
20% SDS
Dissolve 200.0 g SDS in ddH
2
O. Adjust volume to 100 ml with ddH
2
O. It may be necessary
to heat to 65°C to get into solution.
1 M NaHPO
4
pH 7.2
Dissolve 134.0 g Na2 HPO
4
•7H
2
O (F.W. = 268.07) in ddH
2
O. Add 4 ml 85% H
3
PO
4
. Adjust
volume to 1 L with ddH
2
O.
50% Dextran Sulfate
50% dextran sulfate
0.2% sodium azide
Dissolve 50.0 g dextran sulfate and 0.2 g sodium azide in ddH
2
O. Adjust volume to 100 ml
with ddH
2
O. Store at 4°C.
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