Instruction Manual

12
Problem Possible Cause Possible Solution
Bacterial DNA Excessive amount of Determine OD
600
of
contamination bacteria processed culture and do not
exceed 12 OD•ml of
bacteria processed
Excessive agitation of Do not shake or vortex
lysate lysate after addition of
lysis solution
RNA contamination Excessive amount of Determine OD
600
of
bacteria processed culture and do not
exceed 12 OD•ml of
bacteria processed
Compromised RNase Replace kit
activity due to age or
storage conditions
Low A
260/280
Excessive amount of Determine OD
600
of
bacteria processed culture and do not
exceed 12 OD•ml of
bacteria processed
Incomplete suspension Ensure that the bacterial
of bacterial pellet pellet is completely
resuspended prior to
addition of lysis solution
Incomplete mixing of Invert briskly 6–8 times
resuspension, lysis, and after the addition of each
neutralization solutions reagent; check to ensure
homogeneous mixing
Incomplete washing of Use recommended wash
column membranes solution volumes. Add
correct amount of ethanol
to wash solution
concentrate.