User guide

13
Table 2. Cleaning, Regeneration, and Storage Guidelines (continued)
Aminex Aminex Aminex Glyphosate
HPX-87N Column, HPX-87K Column, HPX-87C Column, Analysis Columns,
300 x 7.8 mm 300 x 7.8 mm 250 x 4 mm 100-300 x 7.8 mm
CH
3
CN 30%; CH
3
CN 30%; CH
3
CN 40%; CH
3
CN 30%;
EtOH, IPA, 5% EtOH, IPA, 5% EtOH, IPA, 5% EtOH, IPA, 5%
Na
2
SO
4
K
2
SO
4
Calsium sulfate or KH
2
PO
4
nitrate
MeOH, acids, bases, MeOH, acids, bases, MeOH, acids, bases, Bases, metal ions
30% CH
3
CN in 30% CH
3
CN in 30% CH
3
CN in water 0.005 M Trisodium citrate;
0.01 M Na
2
HPO
4
0.01 M K
2
HPO
4
0.005 M Tetrasodium ethylene
diaminetetraacetate;
4% MeOH, pH 10.0 (NaOH)
0.2 ml/min 0.2 ml/min 0.2 ml/min 0.2 ml/min
25°C 25°C 25°C 50°C
4 hr 4 hr 4 hr 4 hr
(3) run mobile phase
0.020 M Na
2
HPO
4
, 0.020 M K
2
HPO
4
, 0.1 M Ca (NO
3
)
2
0.005 M K
2
HPO
4
,
pH 9 4% MeOH
0.2 ml/min — 0.2 ml/min 0.2 ml/min
85°C 85°C 85°C 50°C
4–16 hr 4–16 hr 4–16 hr 16 hr
0.01 M Na
2
HPO
4
0.01 M K
2
HPO
4
Water 0.005 M KH
2
PO
4
+ 4% MeOH
Lit42D.qxd 11/29/2005 2:23 PM Page 13