User guide

12
Table 2. Cleaning, Regeneration, and Storage Guidelines (continued)
Fermentation Aminex Aminex
Monitoring Column, HPX-72-O Column, HPX-72S Column,
300 x 7.8 mm 300 x 7.8 mm 300 x 7.8 mm
Organic modifier CH
3
CN 40%; CH
3
CN 30%; CH
3
CN 40%;
((
maximum) EtOH, IPA, 5% EtOH, IPA, 5% EtOH, IPA, 5%
Inorganic modifier Phosphoric acid, NaOH, KOH Sulfate salts
nitric acid (<5%)
Avoid MeOH, salts, bases, MeOH, anions different MeOH, anions different
metal ions, amines, than ionic form than ionic form
other organic solvents,
H
2
O >pH 3
Cleaning (1) 5% CH
3
CN in 0.005 (1) 5% CH
3
CN in 0.005 (1) 5% CH
3
CN in 0.005
Solvent M H
2
SO
4
; (2) 30% CH
3
M H
2
SO
4
; (2) 30% CH
3
M H
2
SO
4
; (2) 30% CH
3
CN in 0.005 M H
2
SO
4
CN in 0.005 M H
2
SO
4
CN in 0.005 M H
2
SO
4
Flow rate 0.2 ml/min 0.2 ml/min 0.2 ml/min
Temperature 65°C 25°C 25°C
Duration (1) 4 hr; (2) 12 hr; (1) 4 hr; (2) 6 hr (1) 4 hr; (2) 6 hr
(3) run mobile phase
until steady baseline
Regeneration 0.025 M H
2
SO
4
0.2 M NaOH 0.5 M (NH
4
)
2
SO
4
,
Solvent pH 9
Flow rate 0.2 ml/min 0.2 ml/min 0.2 ml/min
Temperature 65°C 25°C 25°C
Duration 4–16 hr 4–16 hr 4–16 hr
Shipping/storage 0.005 M H
2
SO
4
0.05 M NaOH 0.1 M (NH
4
)
2
SO
4
Solvent
Lit42D.qxd 11/29/2005 2:23 PM Page 12