User guide

Bio-Rad’s columns are tailored for specific applications by optimizing several
parameters, including resin ionic form and column configuration. The following
tables compare the various resin-based analysis columns and provide operating
guidelines. Table 1 provides column specifications and typical operating
parameters. Table 2 provides data which can be useful for resin modification, as
well as cleaning, regeneration, and storage information.
Table 1. Specifications and Operating Guidelines for Aminex Columns
Aminex HPX-87C Aminex HPX-42C Aminex HPX-87P
Column, 300 x 7.8 mm Column, 300 x 7.8 mm Column, 300 x 7.8 mm
Catalog number 125-0095 125-0096 125-0098
Resin ionic form Calcium Calcium Lead
Support Sulfonated divinyl Sulfonated divinyl Sulfonated divinyl
benzene-styrene benzene-styrene benzene-styrene
copolymer copolymer copolymer
Particle size 9 µm 25 µm 9 µm
Maximum pressure 1,500 psi 800 psi 1,500 psi
Maximum flow rate 1.0 ml/min 0.7 ml/min 1.0 ml/min
at temperature max.
Maximum temperature 85°C 85°C 85°C
Typical mobile phase H
2
OH
2
OH
2
O
pH range 5–9 5–9 5–9
Guard cartridge* 125-0128 125-0128 125-0119
Fermentation Aminex Aminex
Monitoring Column, HPX-72-O column, HPX-72S column,
150 x 7.8 mm 300 x 7.8 mm 300 x 7.8 mm
Catalog number 125-0115 125-0141 125-0146
Resin ionic form Hydrogen Hydroxide Sulfate
Support Sulfonated divinyl Sulfonated divinyl Quaternized divinyl
benzene-styrene benzene-styrene benzene-styrene
copolymer copolymer copolymer
Particle size 9 µm 11 µm 11 µm
Maximum pressure 1,500 psi 1,200 psi 1,200 psi
Maximum flow rate 1.0 ml/min 1.0 ml/min 1.0 ml/min
at temperature max.
Maximum temperature 65°C 35°C 65°C
Typical mobile phase H
2
OH
2
OH
2
O
pH range 1–3 9–14 4–9
Guard cartridge* 125-0129 125-0133 125-0132
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