Owner manual

Section 7

Applications

Strong anion exchange resins are used for sample

preparation, enzyme assays, metal separations, and pep-

tide, protein, and nucleic acid separations. The tables

below summarize the applications.

Table 7. Anion Exchange Resins for

Sample Preparation

Application Resin Reference

Recovery of P

from AG 1-X4 Stroop, S. D. and Boyer, P. D.,

glucose-phosphate resin Biochem., 24, 6, 2304 (1985).

Extraction of AG 1-X8 Dombro, R. S. and Hutson, D. G.,

5-hydroxy-indole resin Clin. Chim. Acta, 100, 231 (1980).

acetic acid from

CSF and urine

Anion removal from AG 1-X8 Torben, K. and Penderson, J. S.,

porphyrin in urine resin Scand. J. Clin. Lab. Invest., 38, 279

(1978).

Purification of cyclic AG 1-X8 Shanfield, J., Jones, J. and

nucleotides resin Davidovitch, Z., Anal. Biochem.,

113, 256 (1981).

Ames Keta-Diastix test for glucose

1 M NaCl

Procedure

1. Pack the Poly-Prep chromatography column with

2 ml AG 1-X8 resin, 200-400 mesh, formate form.

2. Convert the resin to the OH form by washing it with

10 bed volumes of 1 N NaOh, then with 5 bed vol-

umes of distilled water.

3. Add 1 ml of the glucose sample.

4. Wash with 3 bed volumes of water.

5. Check for the presence of glucose.

6. Elute the glucose with 1 M NaCl.

7. Check for glucose.

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