Manual

Table of Contents
Section 1 Introduction...............................................................1
1.1 Immunoaffinity Coupling and Usefulness of the
Immobilized Antibody.........................................................1
1.2 Hydrazide Coupling Chemistry...........................................2
1.3 Diagram of Kit Components ...............................................2
Section 2 Monoclonal Antibodies.............................................3
Section 3 Antibody Purification...............................................3
Section 4 Immobilization Protocol...........................................4
4.1 Buffer Exchange..................................................................4
4.2 Oxidation of IgG..................................................................6
4.3 Desalting Procedure.............................................................7
4.4 Coupling of Oxidized IgG to Affi-Gel Hz
Hydrazide Gel......................................................................7
Section 5 Applications of Affi-Gel Hz Immobilized IgG........9
5.1 Conditioning the Immunoaffinity Column..........................9
5.2 Sample Application ...........................................................10
5.3 Elution Suggestions...........................................................10
Section 6 Product Information...............................................11
6.1 Specifications.....................................................................11
6.2 Ordering Information.........................................................11
LIT101C 9/1/98 9:39 AM Page i