User Manual

Chapter 12: Detection............................................................ 30
12.1 SDS-PAGE and Native PAGE Detection ........................................... 30
12.2 Peptide Gel Staining .......................................................... 31
12.3 IEF Gel Staining.............................................................. 31
12.4 Zymogram Gel Staining........................................................ 32
12.5 TBE Gel Staining ............................................................ 32
12.6 TBE-Urea Gel Staining ....................................................... 32
Chapter 13: Blotting ............................................................. 33
13.1 Introduction ................................................................. 33
13.2 Transfer.................................................................... 33
13.2.1 Transfer Buffers .......................................................... 33
13.2.2 Wet Transfer Using the Criterion Blotter ....................................... 33
13.2.3 Transfer Using the Trans-Blot
®
Turbo
™
System.................................. 34
13.2.4 Semi-Dry Transfer Using the Trans-Blot
®
SD Cell ................................ 36
13.3 Total Protein Blot Stains ....................................................... 36
13.4 Immunodetection ............................................................ 37
Chapter 14: Troubleshooting ...................................................... 38
Appendix A: Quick Start Guide .................................................... 40
Appendix B: Buffers ............................................................. 42
Appendix C: Related Literature .................................................... 45
Appendix D: Ordering Information .................................................. 46