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Buffers

B

Running Buffers

10x SDS-PAGE (1 L) 250 mM Tris, 1.92 M glycine, 1% SDS, pH 8.3

(catalog #161-0732)

Tris base 30.3 g

Glycine 144.1 g

SDS 10 g

diH

2

O to 1 L

Do not adjust the pH (~pH 8.3)

10x Native PAGE (1 L) 250 mM Tris, 1.92 M glycine, pH 8.3

(catalog #161-0734)

Tris base 30.3 g

Glycine 144.1 g

diH

2

O to 1 L

Do not adjust the pH (~pH 8.3)

10x Tris-Tricine (1 L) 1 M Tris, 1 M Tricine, 1% SDS, pH 8.3

(catalog #161-0744)

Tris base 121.1 g

Tricine 179.2 g

SDS 10 g

diH

2

O to 1 L

Do not adjust the pH (~pH 8.3)

10x TBE (1 L) 890 mM Tris, 890 mM boric acid, 20 mM EDTA

(catalog #161-0741)

Tris base 107.8 g

Boric acid 55.0 g

EDTA 5.8 g

diH

2

O to 1 L

Do not adjust the pH (~pH 8.3)

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