User Manual

Chapter 4: Native PAGE .......................................................... 14
4.1 Introduction .................................................................. 14
4.2 Criterion Gel Selection Guide for Native PAGE ....................................... 14
4.2.1 Criterion TGX and Criterion TGX Stain-Free Gels.................................. 14
4.2.2 Criterion Tris-HCl and Criterion Stain Free Gels ................................... 15
4.2.3 Criterion XT Tris-Acetate Gels ................................................ 15
4.3 Native PAGE Buffers ........................................................... 15
4.4 Sample Preparation............................................................ 16
4.5 Running Conditions ............................................................ 16
Chapter 5: Criterion Stain Free System .............................................. 17
5.1 Introduction .................................................................. 17
5.2 Criterion Stain Free Workflow .................................................... 18
5.3 Electrophoresis with Criterion TGX Stain-Free Gels .................................... 18
5.4 Using the Gel Doc
™
EZ Imager .................................................. 18
Chapter 6: Peptide Analysis ....................................................... 19
6.1 Introduction .................................................................. 19
6.2 Criterion Tris-Tricine/Peptide Gels ................................................ 19
6.2.1 Gel Composition .......................................................... 19
6.2.2 Gel Selection Guide........................................................ 19
6.3 Tris-Tricine/Peptide Buffers ...................................................... 19
6.4 Sample Preparation............................................................ 20
6.5 Running Conditions ............................................................ 20
Chapter 7: Isoelectric Focusing (IEF)................................................ 21
7.1 Introduction .................................................................. 21
7.2 Criterion IEF Gels ............................................................. 21
7.2.1 Gel Composition .......................................................... 21
7.2.2 Gel Selection Guide........................................................ 21
7.3 IEF Buffers................................................................... 21
7.4 Sample Preparation............................................................ 22
7.5 Running Conditions ............................................................ 22