User Manual

24 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
8.4 Sample Preparation
1. Determine the appropriate protein concentration of your sample based on the detection method
and load volume used. (See Chapter 12 for approximate stain sensitivities.)
2. Dilute 1 part sample with 1 part sample buffer. Do not heat the samples.
8.5 Running Conditions
Power conditions 125 V constant
Starting current 90–120 mA/gel
Final current 35–55 mA/gel
Run time 90 min
Criterion Precast Gels