User Manual

Contents
Chapter 1: Criterion
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Precast Gels................................................... 1
1.1 Introduction ................................................................... 1
1.2 Gel Formulations ............................................................... 2
1.3 Comb Configurations ........................................................... 2
1.4 Specifications ................................................................. 2
1.5 Storage Conditions ............................................................ 3
1.6 Important Notes ............................................................... 3
Chapter 2: Setup and Basic Operation ............................................... 4
2.1 Workflow Overview ............................................................. 4
2.2 Required Materials ............................................................. 5
2.3 Setting Up and Running Criterion Gels in the Criterion Cell ............................... 5
2.4 Removing the Gel .............................................................. 5
Chapter 3: SDS-PAGE............................................................. 7
3.1 Introduction ................................................................... 7
3.2 Criterion Gel Selection Guide for SDS-PAGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.2.1 Criterion
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TGX
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and Criterion
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TGX Stain-Free
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Gels .............................. 8
3.2.2 Criterion Tris-HCl and Criterion Stain Free
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Gels................................... 9
3.2.3 Criterion
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XT Bis-Tris Gels .................................................. 10
3.2.4 Criterion XT Tris-Acetate Gels ................................................ 11
3.3 SDS-PAGE Buffers ............................................................ 12
3.3.1 Running Buffers........................................................... 12
3.3.2 Sample Buffers ........................................................... 12
3.4 Sample Preparation............................................................ 12
3.5 Running Conditions ............................................................ 13