User Manual

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Gel Percentage Optimum Separation Range
XT MES Buffer XT MOPS Buffer
10% 2.5–200 kD 14–220 kD
12% 1–30 kD 6–66 kD
4–12% 2.5–200 kD 10–300 kD
3.2.4 Criterion XT Tris-Acetate Gels
Criterion XT Tris-acetate gels are based on a Tris-acetate buffer system (pH 7.0). It uses discontinuous
acetate and Tricine ion fronts to form moving boundaries that stack and separate large denatured
proteins by molecular weight.
Gel Composition
Gel buffer Tris-acetate, pH 7.0
Crosslinker 3.8% C
Stacking gel 4% T, 3.8% C
Storage buffer Tris-acetate, pH 7.0
Shelf life 8 months at 2–8°C; expiration
date is printed on each cassette
Gel Percentage Optimum Separation range
7% 36–200 kD
3–8% 40–400 kD
Migration charts for protein standards
on Criterion XT Tris-Acetate gels.
Migration charts for protein standards on Criterion XT Bis-Tris gels.
Instruction Manual and Application Guide