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Table 3.1. Criterion precast gels for SDS-PAGE.

Gel Formulation Gels Description

TGX (Laemmli-like) Criterion

™

TGX

™

Laemmli-like, extended shelf life gels

Criterion

™

TGX Stain-Free

™

Laemmli-like, extended shelf life gels with trihalo compounds for rapid

ﬂuorescence detection

Tris-HCl (Laemmli) Criterion Tris-HCl Tris-HCl Laemmli gels

Criterion Stain Free

™

Tris-HCl Laemmli gels with trihalo compounds for rapid ﬂuorescence

detection

Bis-Tris Criterion

™

XT Bis-Tris Based on a Bis-Tris HCl buffer system (pH 6.4); use these gels with

Criterion XT MES buffer for optimum resolution of small proteins

Bis-Tris Criterion XT Bis-Tris Based on a Bis-Tris HCl buffer system (pH 6.4); use these gels with

Criterion XT MOPS buffer for optimum resolution of midsized proteins

Tris-acetate Criterion XT Tris-acetate Based on a Tris-acetate buffer system (pH 7.0)

3.2.1 Criterion TGX and Criterion TGX Stain-Free Gels

Criterion TGX (Tris-Glycine eXtended shelf life) gels are Laemmli-like gels with a proprietary modiﬁcation

that extends their shelf life to 12 months and enhances separation characteristics relative to conventional

gel types. The TGX formulation yields Laemmli-like separation patterns with short run times and

exceptionally straight lanes and sharp bands. TGX gels offer excellent staining quality, greater transfer

efﬁciency, and molecular weight estimation without the need for special, expensive buffers.

These gels are run using standard Laemmli sample buffer and Tris/glycine/SDS running buffer. Two

types of TGX formulations are available:

Criterion TGX — Laemmli-like, extended shelf life gels

Criterion TGX Stain-Free — Laemmli-like, extended shelf life gels with trihalo compounds

that allow rapid ﬂuorescent detection of proteins with the Criterion Stain Free system,

eliminating staining and destaining steps for faster results (see Chapter 5 for more details)

Gel Composition

Crosslinker 2.6% C

Stacking gel 4% T, 2.6% C

Shelf life ~12 months at 2–8°C; expiration date is printed on each cassette

Gel Percentage Optimum Separation Range

7.5% 40–200 kD

10% 30–150 kD

12% 20–120 kD

18% 10–50 kD

4–15% 20–250 kD

4–20% 10–200 kD

8–16% 20–120 kD

10–20% 10–100 kD

Any kD

™

10–200 kD

Any kD is a unique single-percentage formulation that provides a broad separation range and short running time.

Criterion Precast Gels