User Manual

4 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com

Setup and Basic

Operation

2.1 Workflow Overview

Prepare Buffers

Prepare sample and running buffers

Prepare and Load Samples

Dilute in sample buffer

Prepare Gels and

Assemble Electrophoresis Cell

Analyze the Separation

(Chapter 12)

Perform Electrophoresis

SDS-PAGE (Chapter 3)

Native PAGE (Chapter 4)

Peptide Analysis (Chapter 6)

Isoelectric Focusing (Chapter 7)

Protease Analysis (Chapter 8)

Nondenaturing Nucleic Acid PAGE (Chapter 9)

Denaturing Nucleic Acid PAGE (Chapter 10)

2-D Electrophoresis (Chapter 11)

Blot the Gels (Optional)

(Chapter 13)