User Manual

Life Science
Group
11-0677 0911 Sig 02114006166 Rev H US/EG
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Malaysia 60 3 2117 5260 Mexico 52 555 488 7670
The Netherlands 0318 540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700
Russia 7 495 721 14 04 Singapore 65 6415 3170 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700
Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 Thailand 66 2 6518311 United Kingdom 020 8328 2000
Ordering Information
Catalog # Description
Sample Preparation Kits
163-2130 ReadyPrep 2-D Cleanup Kit, 50 preps
163-2089 ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps
163-2088 ReadyPrep Protein Extraction Kit (Membrane 1), 50 preps
163-2087 ReadyPrep Protein Extraction Kit (Signal), 50 preps
163-2086 ReadyPrep Protein Extraction Kit (Total Protein), 20 preps
163-2085 ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps
163-2084 ReadyPrep Protein Extraction Kit (Membrane II), 10 preps
163-2090 ReadyPrep Reduction-Alkylation Kit, 100 preps
163-2100 ReadyPrep Sequential Extraction Kit, 5–15 preps
Buffers and Reagents
163-2094 Bio-Lyte 3/10 Ampholyte, 1 ml
163-2093 ReadyStrip 7–10 Buffer, 1 ml
163-2098 ReadyStrip 3.9–5.1 Buffer, 1 ml
163-2097 ReadyStrip 4.7–5.9 Buffer, 1 ml
163-2096 ReadyStrip 5.5–6.7 Buffer, 1 ml
163-2095 ReadyStrip 6.3–8.3 Buffer, 1 ml
163-2105 ReadyPrep 2-D Starter Kit, 1 kit
163-2106 ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer, 10 ml
163-2103 ReadyPrep Sequential Extraction Kit Reagent 2, 10 ml
163-2104 ReadyPrep Sequential Extraction Kit Reagent 3, 10 ml
163-2083 ReadyPrep 2-D Rehydration/Sample Buffer 1, 10 ml
163-2107 ReadyPrep 2-D Starter Kit Equilibration Buffer I, with DTT, 10 ml
163-2108 ReadyPrep 2-D Starter Kit Equilibration Buffer II, without DTT
or iodoacetamide, 20 ml
163-2129 Mineral Oil, 500 ml
16 3-2111 ReadyPrep Overlay Agarose, 50 ml
163-2092 PROTEAN Plus Overlay Agarose, 125 ml
161-0731 Urea, 1 kg
161-0716 Tris, 1 kg
161-0611 DTT, 5 g
163-2101 Tributylphosphine (TBP), 200 mM, 0.6 ml
163-2109 Iodoacetamide, 30 g
161-0460 CHAPS, 1 g
161-0732 10x Tris/Glycine/SDS, 1 L
161-0772 10x Tris/Glycine/SDS, 5 L cube
PROTEAN
®
i12
™
IEF Cell and Accessories
164-6000 PROTEAN i12 IEF Isoelectric Focusing System, includes basic unit,
electrode assemblies, focusing and rehydration/equilibration trays,
forceps, electrode wicks, mineral oil, cleaning accessories,
IPG strips and sample/rehydration buffer, USB flash drives, styluses
164-6001 PROTEAN i12 IEF Isoelectric Focusing Cell, includes basic unit,
electrode assemblies, and 3 styluses. Focusing trays and other
accessories sold separately.
164-6107 i12 7 cm Focusing Tray, pkg of 1, 7 cm focusing tray, holds up to
twelve 7 cm IPG strips, includes 2 IPG strip retainers for gel-side down
applications, for use with the PROTEAN i12 IEF system
16 4- 6111 i12 11 cm Focusing Tray, pkg of 1, 11 cm focusing tray, holds up to
twelve 11 cm IPG strips, includes 2 IPG strip retainers for gel-side down
applications, for use with the PROTEAN i12 IEF system
16 4- 6113 i12 13 cm Focusing Tray, pkg of 1, 13 cm focusing tray, holds up to
twelve 13 cm IPG strips, includes 2 IPG strip retainers for gel-side down
applications, for use with the PROTEAN i12 IEF system
16 4- 6117 i12 17 cm Focusing Tray, pkg of 1, 17 cm focusing tray, holds up to
twelve 17 cm IPG strips, includes 2 IPG strip retainers for gel-side down
applications, for use with the PROTEAN i12 IEF system
16 4- 6118 i12 18 cm Focusing Tray, pkg of 1, 18 cm focusing tray, holds up to
twelve 18 cm IPG strips, includes 2 IPG strip retainers for gel-side down
applications, for use with the PROTEAN i12 IEF system
164-6124 i12 24 cm Focusing Tray, pkg of 1, 24 cm focusing tray, holds up to
twelve 24 cm IPG strips, includes 2 IPG strip retainers for gel-side down
applications, for use with the PROTEAN i12 IEF system
165-4035 i12 7 cm Rehydration/Equilibration Trays, pkg of 25,
7 cm rehydration/equilibration trays, hold up to twelve 7 cm IPG strips,
for use with the PROTEAN i12 IEF and the PROTEAN IEF systems
Catalog # Description
165-4025 i12 11 cm Rehydration/Equilibration Trays, pkg of 25, 11 cm
rehydration/equilibration trays, hold up to twelve 11 cm IPG strips,
for use with the PROTEAN i12 IEF and the PROTEAN IEF systems
164-6313 i12 13 cm Rehydration/Equilibration Trays, pkg of 25, 13 cm
rehydration/equilibration trays, hold up to twelve 13 cm IPG strips,
for use with the PROTEAN i12 IEF and the PROTEAN IEF systems
165-4015 i12 17 cm Rehydration/Equilibration Trays, pkg of 25, 17 cm
rehydration/equilibration trays, hold up to twelve 17 cm IPG strips,
for use with the PROTEAN i12 IEF and the PROTEAN IEF systems
165-4041 i12 18 cm Rehydration/Equilibration Trays, pkg of 25, 18 cm
rehydration/equilibration trays, hold up to twelve 18 cm IPG strips,
for use with the PROTEAN i12 IEF and the PROTEAN IEF systems
165-4043 i12 24 cm Rehydration/Equilibration Trays, pkg of 25, 24 cm
rehydration/equilibration trays, hold up to twelve 24 cm IPG strips,
for use with the PROTEAN i12 IEF and the PROTEAN IEF systems
164-6040 IPG Strip Retainers, pkg of 2, replacement IPG strip retainers for
use with all sizes of i12 focusing trays
164-6020 i12 Sample Cup Holder, pkg of 1, 12-position sample cup holder,
includes 25 disposable sample cups (#164-6021), for use with the
PROTEAN i12 IEF system
164-6021 i12 Sample Cups, pkg of 25, disposable sample cups, for use with
the PROTEAN i12 IEF system sample cup holder (#164-6020)
164-6030 Gel-Side Up Electrode Wicks, pkg of 100, precut electrode wicks,
for use with the PROTEAN i12 IEF system for gel-side up applications
164-6031 Gel-Side Down Electrode Wicks, pkg of 500, precut electrode wicks,
for use with the PROTEAN i12 IEF system for gel-side down applications
164-6012 Negative Electrode Assembly, pkg of 1, replacement negative
electrode assembly, for use with the PROTEAN i12 IEF system,
can be used with all sizes of i12 focusing trays
16 4- 6011 Positive Electrode Assembly, pkg of 1, replacement positive
electrode assembly, for use with the PROTEAN i12 IEF system,
can be used with all sizes of i12 focusing trays
164-6010 Electrode Assembly Pair, pkg of 1 pair, positive and negative
electrode assemblies, for use with the PROTEAN i12 IEF system,
can be used with all sizes of i12 focusing trays
165-4072 Cleaning Brushes, pkg of 2, cleaning brushes, for cleaning
the focusing and rehydration/equilibration trays used with the
PROTEAN i12 IEF and PROTEAN IEF systems
161-0722 Cleaning Concentrate, 1 kg, concentrated cleaning solution for
use with the PROTEAN i12 IEF and PROTEAN IEF systems.
164-6060 USB Flash Drives, pkg of 2, 2 GB flash drives, compatible with
the PROTEAN i12 IEF system, for transferring data from the
PROTEAN i12 IEF system to a computer for data analysis
164-6050 Styluses, pkg of 3, for use on the PROTEAN i12 IEF system
touch-screen user interface
163-2129 Mineral Oil, 500 ml, for use with the PROTEAN i12 IEF and
PROTEAN IEF systems
165-4070 Forceps, pkg of 1 pair, fine-tipped forceps for handling immobilized
pH gradient (IPG) strips
165-5131 AnyGel
™
Stand, pkg of 1, 6-row gel stand, holds 6 PROTEAN gels,
12 Criterion gels, or 18 Ready Gel
®
mini gels
345-9920 Criterion Staining/Blotting Trays, pkg of 12, gel staining trays,
with lids
PROTEAN IEF Accessories
165-4030 7 cm Focusing Tray, with lid, pkg of 1
165-4020 11 cm Focusing Tray, with lid, pkg of 1
165-4010 17 cm Focusing Tray, with lid, pkg of 1
165-4040 18 cm Focusing Tray, with lid, pkg of 1
165-4042 24 cm Focusing Tray, with lid, pkg of 1
165-4050 Cup Loading Tray, pkg of 1
165-4051 Large Replacement Cups, 150 μl, pkg of 120
165-4052 Small Replacement Cups, 100 μl, pkg of 120
165-4053 Replacement Movable Electrodes, 1 pair
165-4054 Replacement Cup Loading Tray Base
Coomassie is a trademark of BASF Aktiengesellschaft.
Gryphon is a trademark of Datalogic Scanning, Inc.