User Manual

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Fig. 4. Linearity of Oriole stain. A dilution series of carbonic
anhydrase was run on a Criterion gel, stained with Oriole stain,
and imaged on the Molecular Imager VersaDoc MP 4000 imaging
system. Fluorescence associated with the carbonic anhydrase
band was plotted following background subtraction.
Log (fluorescence),
arbitrary units
3.4. Dynamic Range
Oriole fluorescent gel stain has a dynamic range of up to
three orders of magnitude. This allows protein quantitation
in samples of varying concentration over a wide range of
relative abundance.
4.5
4
3.5
3
2.5
2
0 0.5 1 1.5 2 2.5 3
Log carbonic anhydrase, ng