User Manual

NOTE: Use only methanol in preparing staining

solution from the 5 L kit. The use of water, ethanol,

or other solvents will result in poor staining

performance.

Recommended Stain Volume

Volume of Stain

Gel Size Solution per Gel

Ready Gel

or Mini-PROTEAN gel

(8.6 cm × 6.8 cm) 50 ml

Criterion gel (13.3 cm × 8.7 cm) 100 ml

PROTEAN II gel (16 cm × 16 cm or 16 cm x 20 cm) 250 ml

PROTEAN Plus gel (25.6 cm × 23 cm) 500 ml

2.3. Gel Staining

NOTE: Do not ﬁx or wash gel prior to staining.

This will make staining less sensitive.

1. Place gel directly into a clean tray containing the

recommended volume of Oriole ﬂuorescent gel stain.

Cover the tray, place on a rocker or shaker, and agitate

as vigorously as possible without splashing liquid or

damaging the gel.