Instruction manual

Software Reference

Set Quantitive Standard Band...

This allows you to select a band other than the standard.

Display Standard Plot on Log Scale

This allows you to plot Size versus Rf for Protein and DNA gels.

Band Information

This is the data available for analysis and displayed in the band tables. (For a complete discussion of the

Band Tables, refer to that section later in this chapter.) To display a band’s information, double click on its

marker, or click once and select Band Information....

Lane Information

This contains a description of the lane. To display a lane’s information, double click the lane’s number, or

click once and select Lane Information...

• Lane Number: The lane number is displayed at the top of the image.

• Number of Bands: The number of bands in the lane

• Total pmols: The total of all bands in that lane.

• Lane Description: Enter any description for the lane.

Associations...

During automatic band marking, bands in different lanes, having similar migration distances (as measured in

Y-axis pixel counts), are automatically grouped into “Row Associations.” Band associations can be observed

in band tables and in the image display.

To change the sensitivity of this setting, select Associations... from the Bands menu. This changes the

pixel count value used by the software to assign associations. The software then automatically re-defines all

band associations.

Band Associations can be manually adjusted one band at a time by clicking on the band value in any band

table and, while holding down the left mouse button, moving the data to the desired location. Band associa-

tions can also be manually adjusted in the Image window by clicking and dragging the band to its new asso-

ciation. When a band is clicked on and dragged, a Linking Cursor appears. When you move it, the cursor

changes to an Up or Down arrow until you release the mouse button.

To remove a band from a band association, click on it and drag it from its row. If you drag to the top or bot-

tom of the image, a new row is created in the band table, with the band placed in that row.