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10
Installation and Startup
1.4 IMAGE ANALYSIS USING THE IMAGE PROVIDED WITH YOUR SYSTEM
The key steps to analyzing an image are:
• Open the image file (Use the image file demo1.RAW)
• Adjust the image display
• Find bands
• Perform a standard lane analysis
• Save the data file
To analyze the sample image provided with your system, follow the procedure below.
1. Open an image file.
a. Select the Open command from the Files menu.
b. From the Open File dialog box, select the image file demo1.RAW that has been provided.
c. Press the OK button to open the file.
2. Adjust the Image display’s Contrast and Brightness.
The Image Histogram, shown in Figure 7, offers the following controls:
• Contrast
• Brightness
• Autoscale, for automatically setting Contrast and Brightness.
3. Locate bands using Glyco Doc’s automatic band location functions.
These functions find all bands and mark with a + those bands whose data is displayed in the Band
Tables.
a. From the Bands pull-down menu, select either of the following:
• To locate bands in a region, create a Marking Rectangle. Then, select Find Bands In Marked
Rectangle.
When defining a region, do not “crowd” the boundaries of a lane; include a small bit of the
region beyond the band’s edge.
• To find bands in the entire image, select Find Bands In Complete Image.
b. Verify the number of lanes in the image area.
Glyco DOC displays the number of lanes it has identified; if this number is not correct, enter the cor-
rect number.
Note: When imaging the complete gel, not all lanes may be displayed; to see more lanes, pull the
image window’s border to the right.
c. Bands selected for analysis are automatically indicated by a + marker.
To view all detected bands, select Show Band Outlines from the Options menu.
4. Increase/decrease the number of bands marked for analysis.
a. From the Bands menu, select Find More Find Fewer bands.
b. Using the Find More or Less Bands window, point and click on the appropriate scroll bar arrow to
quickly mark or unmark bands within the entire image.
c. Press OK.