Instruction manual

System Operation

Table 6.

Manual Mode Operation: Pump

Reference Description

Buffer screen: Selecting Buffer from the Main Menu screen

displays the Buffer screen, shown to the left. This screen

allows you to select the buffer to be delivered to the column.

Use the Prev and Next keys to select a single buffer (A through

E) or a mixture of buffers A and B. To set a mixture, select the

Mix soft key and enter the percent of buffer B. Note: If a valve

is not connected on the BioLogic LP, its letter or letters appear

in lower case and cannot be selected.

5.2.2 Manual Mode Operation of the Fraction Collector

Table 7 discusses each of the functions available in Manual mode operation of the fraction collector. An

overview of the Fraction Collector’s Manual mode operation is shown in Figure 16.

Table 7.

Manual Mode Operation: Fraction Collector

Reference Description

To begin Manual mode operation of the fraction collector:

a. From the Mode keys select Manual.

b. From the Instrument keys select Collector.

c. The main menu for manual mode operation of the fraction

collector is shown below.

The display shows the following:

• Fraction Size: The fraction size can be specified as a vol-

ume (ml) or time (minutes).

• UV: The current UV reading.

• Diverting to Waste/ Collect: Indicates the position of the

SV-3 Diverter valve.

• Cond: The current Conductivity reading.

Description of the soft key choices follows.

Method: <<name>> Manual Operation

Buffers: M [A] B C D E

(Select MIX for Mixture of A and B)

MIX CANCEL OK

M4a

Method: <<name>> Manual Operation

Fx Size: 1.00 ml UV: 0.0111 AU

Diverting to Waste Cond: 0.000 mS

START F/X SIZE ADVANCE MODEL

M3M1

M2 M4

Manual

Collector