Product Manual

ManualsBrandsBeckman Coulter ManualsLab & PhlebotomyCOULTER LYSE S III diff Lytic Reagent

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For In Vitro Diagnostic Use

INTENDED USE

For use as an erythrocyte lytic agent for the quantitative

determination of hemoglobin and for leukocyte counting

and sizing on COULTER HmX, LH 500, LH 750, and

LH 780 hematology analyzers. Use with only ISOTON III

or LH Series diluent. Refer to your instrument product

manuals and/or online help, as applicable.

SUMMARY

COULTER LYSE S III diff Lytic Reagent is used as a lytic

agent to prepare a diluted whole-blood sample for the

quantitative determination of hemoglobin, the enumeration

of leukocytes (white blood cells), and the derivation of

leukocyte subpopulations.

PRINCIPLES

COULTER LYSE S III diff Lytic Reagent is a clear,

aqueous solution containing potassium cyanide and

quaternary ammonium salts.

When the lytic reagent is added to a diluted whole-blood

sample, the surface-active properties of the quaternary

ammonium salts destroy the red cell membranes, thus

lysing the red blood cells (stromatolyzation) and releasing

the hemoglobin. The released hemoglobin chemically

reacts with potassium cyanide to form a stable

hemochromagen that is quantitatively measured at an

absorbance wavelength.

As the lytic reagent is destroying the red cell membranes

to release the hemoglobin, it reduces the size of the

resulting cellular debris to a level that does not interfere

with the leukocyte (white blood cell) counts. On COULTER

hematology analyzers with histogram differential, the lytic

reagent causes differential shrinkage of the leukocytes

into predictable volume components, namely lymphocytes,

mononuclear cells, and granulocytes. Additionally, the

reaction of the quaternary ammonium salts functions to

reduce the amount of protein buildup in the sensing

orifices of the instrument.

REACTIVE INGREDIENTS

Quaternary Ammonium Salts……………………..19-26 g/L

Isopropanol............................................................1-2%

Potassium Cyanide...............................................0.15-0.5 g/L

WARNINGS

 Product contains <2% Isopropyl Alcohol and

<2% Quaternary Ammonium Salts. Irritating to eyes.

 Do not inhale and/or ingest.

 In case of contact with eyes, rinse immediately with

plenty of water and seek medical advice.

 DO NOT REUSE CONTAINER.

STORAGE, STABILITY, AND DISPOSAL

 Store COULTER LYSE S III diff Lytic Reagent at

2-30°C.

 Do not use product past expiration date.

 Use product at temperatures stated in the instrument

product manuals and/or online help.

 Opened containers are stable for 60 days when stored

as recommended.

 Dispose of waste product, unused product, and

contaminated packaging in compliance with federal,

state, and local regulations.

PREPARATION

COULTER LYSE S III diff Lytic Reagent is ready to use.

Replace the reagent as directed in your instrument

product manuals and/or online help.

IMPORTANT: If product has been partially or completely

frozen, allow product to warm to room temperature. Mix

product by gentle inversion prior to placement on the

instrument. Install and prime the reagent as directed in

your instrument product manuals and/or online help. Verify

background counts are acceptable before analyzing

patient samples.

PRODUCT AVAILABILITY

COULTER LYSE S III diff Lytic Reagent - 1 x 1 L

8546983

COULTER LYSE S III diff Lytic Reagent - 1 x 5 L

8546796

TRADEMARKS

Beckman Coulter logo, COULTER and LYSE S are

trademarks of Beckman Coulter, Inc.; Beckman Coulter,

COULTER and LYSE S are registered in the USPTO and

SIPO.

For additional information, or if damaged product is

received, call Beckman Coulter Customer Service at

800-526-7694 (USA or Canada) or contact your local

Beckman Coulter Representative.

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821

www.beckmancoulter.com

Printed in USA

Made in USA

COULTER

LYSE S

III

diff

Lytic Reagent

8546983

8546796

PN 772260-AB