Item Brochure

Serum
BD Vacutainer
®
Plus Plastic Serum Tubes have spray-coated silica and are used for
serum determinations in chemistry. Samples processed in these tubes may also be
used for routine blood donor screening, immunohematology, and diagnostic
testing of serum for infectious disease.*
Closure
Reference #
Tube Size Draw Volume
Type Colour Label type
(mm) (mL)
366434 G 13x75 5.0 P
366668 13x75 3.0 P
367812 ✓ 13x75 4.0 P
367814 13x100 5.0 P
367815 ✓ 13x100 6.0 P
366430 G 16x100 10.0 P
367820 ✓ 16x100 10.0 P
* The performance characteristics of these tubes have not been established for infectious disease testing in general;
therefore, users must validate the use of these tubes for their specific assay-instrument/reagent system
combinations and specimen storage conditions.
DISCARD (No additive)
366703 13x75 3.0 Clear P
366408 13x100 6.0 Clear P
▲ BD CLINICAL ADVANTAGE
According to CLSI, drawing a discard volume is required for the following circumstances:
- When using a winged blood collection set for venipuncture and a coagulation (citrate)
tube is the first specimen tube to be drawn, a discard tube should be drawn first. The
discard tube must be used to fill the blood collection set tubing’s “dead-space” with
blood but the discard tube does not need to be completely filled. This important step will
ensure maintenance of the proper blood-to-additive ratio of the blood specimen. The
discard tube should be a nonadditive or coagulation tube.
- When a line has been flushed with a solution, it is necessary to clear the line of this fluid
before drawing a specimen. The volume discarded depends on the dead-space within the
line and is equivalent to 2 times the dead-space volume for noncoagulation tests, and 5
mL or six times the dead-space volume for coagulation tests.
1
1
CLSI, Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture - Sixth Edition; Approved
Standard. CLSI documentation H3-A6, Vol. 27, No. 26, Oct. 2007.
EDTA
BD Vacutainer
®
spray-coated K2EDTA Tubes are used for whole blood
hematology determinations, immunohematology and blood donor screening.*
Closure
Reference #
Tube Size Draw Volume
Type Colour Label type
(mm) (mL)
367841 ✓ 13x75 2.0 P
367856 ✓ 13x75 3.0 P
367861 ✓ 13x75 4.0 P
367844 ✓ 13x75 4.0 P
367863 13x100 6.0 P
366643 16x100 10.0 ST
367899 ✓ 13x100 6.0 P**
* The performance characteristics of these tubes have not been established for infectious disease testing in general;
therefore, users must validate the use of these tubes for their specific assay-instrument/reagent system
combinations and specimen storage conditions.
** "Crossmatch" label designed for American Association of Blood Banks (AABB) patient identification.
▲ BD CLINICAL ADVANTAGE:
K2EDTA is the “anticoagulant of choice in specimen collection and blood cell
counting” according to both CLSI (formerly NCCLS) and the International Council for
Standardization in Haematology (ICSH)
1,2
. It was chosen for the following reasons:
1) K3EDTA results in greater RBC shrinkage;
2) K3EDTA produces a larger increase in cell volume on standing; and
3) K3EDTA is a liquid additive and will result in the dilution of the specimen.
Results of directly measured values can be 1-2% lower than results with
K2EDTA. K2EDTA is sprayed onto the interior of the plastic tube and like
K3EDTA requires 8-10 inversions for thorough mixing of blood with
the anticoagulant.
1
NCCLS, Evacuated Tubes and Additives for Blood Specimen Collection – Fifth Edition; Approved Standard. NCCLS
documentation H1-A5, Vol. 23, No. 33, Dec. 2003.
2
International Council for Standardization in Haematology Expert Panel on Cytometry. Recommendation of the
International Council for Standardization in Haematology for ethylenediaminetetraacetic acid anticoagulation for
blood cell counting and sizing. Am J Clin Pathol 1993; 100:371-372.