User manual

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8. On and off-line calculation and plotting of several waveform parameters including:
a. DC Baseline
b. Peak Amplitude
c. Latency
d. Slope and Maximum Slope
e. Area
f. Duration
g. Rise Time
h. Decay Time
i. Coastline
j. PopSpike Amplitude
k. PopSpike Latency
l. Average Amplitude
m. Cell Input Resistance (Rm)
n. Patch Electrode Series Resistance (Rs)
9. Analyze all S0- and S1-evoked postsynaptic responses in both AD channels in a sweep
10. Special analyses of trains including:
a. Analyze every pulse in train, but use the baseline of the first pulse as the baseline for each pulse
b. Analyze whole train by analyzing only first pulse in train but detecting whole train
c. Analyze train using baseline of the first pulse and response of the last pulse
11. Automatic blanking of stimulus artifacts to allow accurate determination of peaks and areas in a
train
12. Measurement of Patch Electrode Series Resistance (Rs) using Rs peak, or Rs single or double
exponential curve fitting
Simpler WinLTP functionality includes:
1. WinLTP records synaptic activity in extracellular, intracellular or patch clamp modes
2. 2 AD channel acquisition (down to 25 usec sample interval)
3. Two extracellular stimulation outputs (S0 and S1)
4. Two simultaneous patch-clamp recordings using two analog outputs
5. Analog stimulation including analog trains and ramps (loop within loop stimulation)
6. Repetitive sweeps with simultaneous data acquisition (up to 1,000,000 samples and 100 sec) and
stimulation (using two extracellular pathway stimulation, S0 and/or S1, and epoch-like digital and
intracellular analog stimulation).
7. The protocols for the basic LTP experiment are either slow single pathway S0 stimulation, or slow
alternating dual pathway (S0 then S1) stimulation.
8. The sweep data can be signal averaged and digitally filtered on-line and off-line
9. LTP induction can be produced by:
a. Single train
b. Repetitive train (theta burst stimulation)
c. Primed burst stimulation (limited implementation)
10. LTD stimulation and analysis can be performed using fast repetitive sweeps (at up to 10 Hz) with
no delay between sweeps, or a single sweep lasting several minutes for faster repetitive
11. Patch sealtest protocol implemented
12. Save an ADsweep graph as a Windows Enhanced Metafile
13. Save your Spreadsheet/ AmpFile data to an Excel XLS file
14. Reanalyze straight ASCII files (skip header)
15. Automatic data folder creation at start-up
16. Change the internal National Instruments board gain
17. WinLTP Reanalysis works on Macs with Intel processors
18. On-line acquisition runs in Windows XP, Vista, 7 and 8 (64- and 32-bit, M- and X-Series boards)
and Windows 2000, XP, 7 and 8 (32-bit only, Digidata 132x boards)