User manual

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4.11 Setting the Calculation Detection Criteria .................................................................................. 100
4.12 Analyzing All S0- and S1-Evoked Postsynaptic Responses in Both AD channels in a Sweep ... 115
4.13 Special Analyses of Trains ........................................................................................................ 118
4.14 Saving the Protocol File to Disk ................................................................................................. 121
4.15 Run the Experiment .................................................................................................................. 122
4.16 AutoCreate a new Data Folder (using CTL-F) while running an experiment .............................. 125
4.17 Saving AMP and XLS Analysis Files After an Experiment ......................................................... 125
4.18 Getting a quick printout of your Analysis Graphs using a PrintScreen program ......................... 129
4.19 Compress Data Files at the End of an Experiment? .................................................................. 129
4.20 Recovering from a Non-Fatal Bug or a Crash ............................................................................ 130
4.21 How to Copy Over a Protocol File from an Old WinLTP Program to a New WinLTP Program ... 133
CHAPTER 5 – VIEW AND IMPOSE SINGLE AND AVERAGED SWEEPS ........................................... 134
5.1 Viewing Sweeps ......................................................................................................................... 135
5.2 Viewing Sweeps by Clicking on an Analysis Graph Data Point ................................................... 137
5.3 Imposing Single Sweeps ............................................................................................................ 139
5.4 Imposing Averaged Sweeps ....................................................................................................... 141
5.5 Changing Filtering Affects All Viewed and Averaged/Imposed Sweeps ...................................... 143
CHAPTER 6 – PATCH-CLAMP SEALTEST PROTOCOL ..................................................................... 145
6.1 Electrode in Bath ........................................................................................................................ 145
6.2 Forming a Seal ........................................................................................................................... 146
6.3 Going Whole Cell ....................................................................................................................... 147
CHAPTER 7 – PROTOCOL BUILDER – CIRCULAR SCRIPTING ........................................................ 148
7.1 The Protocol Builder in Basic, Standard and Advanced Modes .................................................. 148
7.2 Inserting and Deleting Protocol Lines ......................................................................................... 149
7.3 Rules of Protocol Building .......................................................................................................... 150
7.4 Examples of Circular Scripting ................................................................................................... 150
CHAPTER 8 – PROTOCOL BUILDER – SEQUENTIAL SCRIPTING .................................................... 161
8.1 Circular Scripting to Sequential Scripting ................................................................................... 161
8.2 Sequential Scripting ................................................................................................................... 163
8.3 Sequential Scripting with Perfusion Changes ............................................................................. 164
CHAPTER 9 – PROTOCOL LINKING ................................................................................................... 165
9.1 Using Protocol Linking to extend Circular and Sequential Scripting ............................................ 165
9.2 Linking to the Next Protocol File ................................................................................................. 166
9.3 Saving the Continuous Acqusition Files in Reasonable Durations .............................................. 167
9.4 Extending the Run-Time of the MainProtocol ............................................................................. 168
CHAPTER 10 – AUTOMATED EXPERIMENTS USING AUTOMATED PERFUSION CONTROL ......... 169
10.1 Automated Perfusion Control ................................................................................................... 169
10.2 Automated Multi-Slice Extracellular Experiments ..................................................................... 170
10.3 Automated Single-Line Perfusion Patch-Clamp Experiments ................................................... 190
10.4 Automated Dual- and Triple-Line/Stepper Perfusion Patch-Clamp Experiments....................... 206
CHAPTER 11 – EXPERIMENTAL LOG ................................................................................................. 215
11.1 Capabilities of the Experimental Log ........................................................................................ 215
11.2 Use the PrintToLog tabsheet to control printing of useful information ....................................... 217
11.3 Printing AvgLoops is usually not useful .................................................................................... 217
11.4 Print only sweeps that output important induction stimulation ................................................... 219