User manual

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WinLTP can convert many WinLTP ADsweep files to:
1) Many single-sweep gap-free *.ABF files
2) Many single-sweep episodic *.ABF files
3) One multi-sweep episodic *.ABF file
Both the gap-free and episodic ABF files are in the binary integer file format, not floating point format.
The version of these ABF files is the ‘lowest common denominator’, Version 1 (used by Molecular
Device’s pClamp 8 and 9) so as to be accessed by the largest number of analysis programs. WinLTP
does not convert to ABF Version 2 files (used by pClamp 10). However, pClamp 10 can convert Version
1 ABF files to Version 2.
Also, it is important to realize that the stimulation information in the header of WinLTP ADsweep files
is not transferred into the ABF files. In other words, the converted ABF files contain no stimulation
information. This is because of substantial differences in the stimulation capabilities of WinLTP and
pClamp. However, this should usually not be too much of a problem when cursors are used to select
sections of the sweep for analysis.
Conversion to ABF files can either be performed in the Online/Acquisition program either in the Demotrial
Period or with a Temporary or Permanent Licence Key File to change it to the Advanced Mode, or in the
Reanalysis program with a Permanent Licence Key File.