User manual

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15.12 Improved Interaction with Other Data Acquisition and
Analysis Programs
There has also been an effort to improve interaction with other data acquisition and analysis programs:
1. WinLTP can now reanalyze ASCII sweep files (with or without a header) (Section 15.12.1).
Therefore ASCII sweep files from any program can be analyzed with WinLTP.
2. In www.winltp.com there are also two utility program that can convert multisweep ASCII files into
many single sweep ASCII sweep files that can be imported into WinLTP for reanalysis:
a. Atf2swps.exe converts an Axon Text file (*.ATF) such as generated by Axon Instruments'
AxoScope or pClamp's Clampex program into many single sweep ASCII files that can be
imported into WinLTP for reanalysis. Therefore WinLTP can also indirectly access gap-free,
episodic stimulation and fixed-length event driven Axon Binary Files (*.ABF) after they are
converted to an Axon Text File.
b. Dv2swps.exe converts a multisweep ASCII file exported by DataView (a program written by W.J.
Heitler) into many single sweep ASCII files that can be imported into WinLTP. Because
DataView can convert tape recorder type files such as gap-free Axon Binary Files (*.abf), CED
Signal (*.cfs) files, CED Spike2 (*.smr) files, ASCII text files, or Raw binary files, WinLTP can
indirectly access these files as well, and can analyse them using the LTP Program's particular
analysis capabilities.
3. In addition, WinLTP can also save reanalyzed ASCII sweep files (Section 15.12.2). This is useful
for saving ASCII sweep files obtained by other acquisition programs where WinLTP can be used to
remove stimulus artifacts, average several sweeps, or filter the sweeps.
15.12.1. Reanalyzing ASCII Sweep Files
The WinLTP Reanalysis program can also reanalyze general ASCII ADsweep files (Fig. 15.12.1.1.). This
figure shows the analysis of two EPSC Peak Amplitudes, and the patch electrode series resistance (Rs)
and the cell input resistance (Rm). Because there is no stimulation information in a general ASCII
ADsweep file, the WinLTP has to provide this stimulation information. It does this by using a 'stimulus
template' and choosing the source of stimulation information from Screen Fields rather than the Disk
File (see Section 15.5).
To set the ADsweep File Type, use the AmpFile menu command (Fig. 15.4.3):
AmpFile -> Set reanalysis ADsweep file type...
This calls up the Reanalysis ADsweep File Type dialog box (Fig 15.12.1.2). This allows you to change
from the default WinLTP’s ASCII file format (see Fig. 16.1.1) to 1) one data column ASCII, 2) two data
columns ASCII, 3) a time column (on the left) plus one data column ASCII, or 4) a time column (on the
left) plus two data columns ASCII.