User manual

201
DigitalOut Bits
3 2 1 0
Ch 1 0 0 0 0
Ch 2 0 0 0 1
Ch 3 0 0 1 0
Ch 4 0 0 1 1
Ch 5 0 1 0 0
Ch 6 0 1 0 1
Ch 7 0 1 1 0
Ch 8 0 1 1 1
Ch 9 1 0 0 0
Ch 10 1 0 1 0
Ch 11 1 0 0 1
Ch 12 1 0 1 1
Ch 13 1 1 0 0
Ch 14 1 1 0 1
Ch 15 1 1 1 0
Ch 16 1 1 1 1
Table 10.3.1.2.3. Setting the DigitalOut bits 3 to 0 in binary to turn on Ch1 to 16 on the AutoMate
Scientific ValveLink 8.2 controller. To turn all channels off has to be done manually.
The Warner VC-8 controller and the ALA Scientific VC
3
8 cannot be binary controlled by 4 binary bits.
Channels 1, 2 3 and 4 can also be controlled ’linearly’ or independently with the high-speed digital output
from Port0 by setting one bit for each channel (Table 10.3.1.2.4). No bits on means all channels are off.
This works when using the ALA Scientific, Automate Scientific, BioScience Tools and Warner controllers.
DigitalOut Bits
3 2 1 0
All Chs Off 0 0 0 0
Ch 1 0 0 0 1
Ch 2 0 0 1 0
Ch 3 0 1 0 0
Ch 4 1 0 0 0
Table 10.3.1.2.4. Setting the DigitalOut bits 3 to 0 to turn on ‘linear’ Channels Ch ‘1’ to ‘4’.
Note that if your antagonist does not rapidly unbind, and therefore agonist solutions need not contain
antagonist, it is strongly preferable to use AO1 rather than Digital Output for fast perfusion changes during
a sweep. This is because if you enter a -1V into the Amplitude field of the epoch, that means that the
current fast perfusion channel will continue to be output during sweep epochs containing a -1V, and
therefore each sweep can contain a unique agonist concentration, thereby limiting the number of different
sweep stimulations required. This capability is not, to our knowledge, present in Molecular Devices
pClamp or in HEKA’s PatchMaster.
In addition, setting binary digital outputs is not nearly as straight forward as setting voltages as channel
numbers for analog output.
For WinLTP 2.10, which has 4 different PxSweeps (P0, P1, T0 and T1), and when the antagonist is tightly
bound, you can deliver 16 different antagonist concentrations and 4 agonist concentrations in one
protocol file (when using -1V and analog output). When the antagonist is loosely bound and the agonist