User manual

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Antagonist Agonist
Ch 1 1
Ch 2 2
Ch 3 3
Ch 4 1 1
Ch 5 1 2
Ch 6 1 3
Ch 7 1 4
Ch 8 2 1
Ch 9 2 2
Ch 10 2 3
Ch 11 2 4
Ch 12 3 1
Ch 13 3 2
Ch 14 3 3
Ch 15 3 4
Table 10.3.1.1.2. If the antagonist does rapidly unbind, 15 solutions are required to produce a dose-
response curve for 4 agonist concentrations and just 3 antagonist concentrations. This requires two eight
channel, eight valve controllers, or one 16 channel, 16 valve controller.
10.3.1.2 Control of Fast0 Perfusion by Analog and Digital Output
To use the Single-Line Fast Perfusion, choose the ‘Fast0 Perfusion Change During and Between Sweeps’
in the automated perfusion control radiobutton group (Fig. 10.3.1.2.1). You first have to decide how you
want to use AO1 in the ‘Use AO1 For ’ radiobutton group. If you do not need to use AO1, then Fast0
Perfusion can use it as well as the thigh 4-bits on the high-speed digital output Port0. Then you can have
the following choices (Fig. 10.3.1.2.1A):
1) Controlling 8 channels using AnalogOut 1 (for the ALA Scientific and Automate Scientific and
Warner controllers),
2) Controlling 16 channels also using AnalogOut 1 (for the BioScience Tools controller),
3) Controlling 16 channels using AnalogOut 1 and AnalogOut 2 (for the Automate Scientific, ALA
Scientific, BioScience Tools and Warner controllers)
4) Controlling 4 channels using 4 bits on the high speed digitial output Port 0 (for Automate, ALA,
BioScience Tools and Warner controllers)
5) Controlling 15 channels using 4 bits on the high speed digitial output Port 0 (for the BioscienceTools
controller), where if all the bits are off, all channels are off.
6) Controlling 16 channels using 4 bits on the high speed digitial output Port 0 (for the Automate and
ALA controllers), where if all the bits are off, Channel 1 is on.
If you are using AnalogOut 1, you obviously cannot be using it for a second patch-clamp stimulation
channel. And if you are using AnalogOut 2, you need a National Instruments board that has 4 rather than
just 2 analog outputs such as the PCI-6229, PCIe-6323 or USB-6343 BNC. The current version of
WinLTP (2.10) does not check to see that AnalogOut 2 is present – if it is not present, it just won’t write to
it.
If you do need to use AO1 for something other than fast perfusion, then Fast0 Perfusion cannot use it can
only use the high 4-bits on the high-speed digital output Port0. Then you can have the following choices
(Fig. 10.3.1.2.1B):