User manual

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Fig 10.3.1.1. Standard perfusion system setup for single cell, single-line patch-clamp experiments. The
crucial dead volume in this case is from the manifold to the tip of the perfusion pipette (shown in dark
purple). The main difference between this and the slice standard perfusion system (Fig. 10.2.4.1A) is the
use of high-speed valves rather than the slower pinch valves.
The Fast Perfusion changes can be controlled by both the Perfuse statement in the Protocol Builder to
change between sweeps, and by changing either digitial or analog stimulation during the sweep to change
perfusion during the sweep.