User manual

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your perfusion system in the pre-flush manner below the water bath, and use the rest of your setup as
normal.
Or to start out with, you may first wish to increase the number of slice wells by adding a laminar perfusion
chamber on top of your current chamber bath. For optics, you can just swing your microscope from one
chamber to another.
Your ultimate systems may look like those in Fig. 10.2.9.1. Fig 10.2.9.1A shows 2 chambers (each
controlled by separate perfusion systems (either automated or manual) and by separate
WinLTP/ADboard. There are two slices (one slice per well) obtained from the same animal, but will be
able to undergo two completely separate experiments. Each slice has one stimulating and one recording
electrode, although in the two chamber system (Fig. 10.2.9.1A), it would be easy to increase the recording
electrodes to two. Fig. 10.2.9.1B shows 3 chambers with one well/per chamber, and one slice/well. Fig.
10.2.9.2 shows 4 chambers with one well/per chamber, and one slice/well. This is realistically the
maximum number of perfusion chambers, slices and manipulators under one microscope.
If you want to prepare slices from 2 animals, you could use the configuration shown in Fig. 10.2.9.3.
Here, the two chambers (in purple or orange) are each controlled by separate perfusion systems and ene
WinLTP/ADboard. There are two wells per chamber, and each well contains one slice from a different
animal.
Alternatively, if you wish to experiment on many slices from one animal using one experimental protocol,
you could have (in future versions of WInLTP) the configuration shown in Fig. 10.2.9.4. In this case, 1
chamber is controlled by 1 perfusion system and one WinLTP/ADboard, but there are 4 wells per
chamber. Note that the current WinLTP 2.10 only has 2 AD channels, but shortly a 2.xx version will have
5 AD channels.