User manual

183
MainProtocol
[x] Slow0 Perfuse [ 1] ACSF
[ ] Sub0 [99999]
[x] Slow0 Perfuse [ 2] 100uM AP5
[x] Sub0 [ 2]
[x] T0sweep [ 2]s
[x] Sub0 [ 5]
[x] Slow0 Perfuse [ 1] ACSF
[x] Sub0 [ 4]
[x] T0sweep [ 2]s
[x] Sub0 [ 6]
EndProtocol
[x] SubProtocol0 [y] [ ]
[x] Loop [ y]
[X] P0sweep [ 2]s
EndLoop
EndSubProtocol
Fig 10.2.7.5. In the future, the Sequential Perfusion Protocol will look much clearer with SubProtocols
added.
The protocol in Fig. 10.2.7.5 is the same protocol in Fig. 10.2.7.2B except that the repetitive
[x] Loop [ y]
[X] P0sweep [ 2]s
EndLoop
Lines are placed once in the SubProtocol0 rather than repeated six times in the MainProtocol.
So in LTP (or similar) experiments with this automated perfusion control, once you determine the correct
extracellular stimulation strength, and once you unclick the Baseline Loop once the baseline is stable
(Figs. 7.1.1.1D and 10.2.7.2B), all stimulation and perfusion changes will be automatically performed –
and you can go off and read a paper in peace, reanalyze yesterday’s data in peace, run another slice, or
go the pub.
10.2.8 Running Many WinLTP Programs Simultaneously (on one computer)
In addition to Sequential Protocol Scripts and automated perfusion control, the other important capability
of WinLTP to do Automated Multi-Slice Experiments is the ability to realistically Run Many WinLTP
Programs At Once (Fig. 10.2.8.1).
Strictly speaking, the ability to realistically Run Many WinLTP Programs At Once is not needed if you are
running many slices from one animal or one slice from many animals so that all the slices are exposed to
the SAME stimulation and perfusion solutions.