User manual

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The experimental log output for the protocol running in Fig. 10.2.7.2 is shown in Fig. 10.2.7.3. Each
perfusion change is clearly shown by time, channel number and channel label (eg “Ch2 100um AP5”),
and by then next sweep following the perfusion change.
13:36:06.6 0.0 Start MainProtocol
13:36:06.6 0.0 Slow0 Perfuse Ch 1 ACSF is on
13:36:07.1 0.5 Slow0 Perfuse Ch 1 ACSF
13:36:07.1 0.5 Enter Loop0
13:36:07.1 0.5 1D200057.P0, First Loop sweep
13:38:07.1 2:00.5 Leave Loop0
13:38:07.1 2:00.5 Slow0 Perfuse Ch 2 100uM AP5
13:38:07.1 2:00.5 Enter Loop0
13:38:07.1 2:00.5 1D200068.P0, First Loop sweep
13:40:07.1 4:00.5 Leave Loop0
13:40:07.1 4:00.5 1D200076.T0, Next sweep after end of Loop
13:40:22.1 4:15.5 Enter Loop0
13:40:22.1 4:15.5 1D200077.P0, First Loop sweep
13:41:07.1 5:00.5 Leave Loop0
13:41:07.1 5:00.5 Slow0 Perfuse Ch 1 ACSF
13:41:07.1 5:00.5 Enter Loop0
13:41:07.1 5:00.5 1D200080.P0, First Loop sweep
13:43:07.1 7:00.5 Leave Loop0
13:43:07.1 7:00.5 1D200088.T0, Next sweep after end of Loop
13:43:22.1 7:15.5 Enter Loop0
13:43:22.1 7:15.5 1D200089.P0, First Loop sweep
13:45:22.1 9:15.5 Leave Loop0
13:45:22.1 9:15.5 Stop MainProtocol
Fig. 10.2.7.3. The experimental log output for the protocol running in Fig. 10.2.7.2.
Then go to the Protocol Builder (left side of Fig. 10.2.7.2B), click on the ‘Perfuse’ button with the left
mouse button, drag down the mouse cursor to the appropriate between-line location in the Sequential
Protocol Script, and release the left mouse button to insert the ‘Perfuse’ statement in the Sequential
Protocol Script (see red lines).
Then click on the Perfuse Channel Number field and increment/decrement to the desired Channel
Number. Note that the Perfusion Label to the right of the number field is the Perfusion Label set in the
Perfuse tabsheet.