User manual

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Fig 10.2.4.1. Standard, pre-flush and continuous-to-waste slice perfusion systems. A) The slice standard
perfusion system with 1 valve/line. B) The slice pre-flush perfusion system with 2 valves/line for a four
line perfusion system. (The Flush Valve has been turned on for a sufficient time to clear the perfusion line
from the reservoir to the T-fitting, but the Chamber Valve has not yet been turned on). The dead volumes
for the two system lines are indicated in red. Basically the pre-flush system has a dead volume from the
T-fitting to the manifold, which could be approximately 10 cm or less. The dead volume of the standard
perfusion system could be anywhere from almost 0 cm to 1 meter depending on your configuration. C) A
slice continuous-to-waste perfusion system using a Normally Open (NO) – Normally Closed (NC) pinch
valve to flush the tubing between the reservoir and the valve. The pinch valve controlling the solution flow
between the ‘Y’ connector and the manifold is Normally Closed, and between the ‘Y’ connector and waste
is Normally Open, and the tubing between the reservoir and the ‘Y’ connector is aerated. When the
perfusion channel is turned on, the continuous flow to waste stops, and aerated solution flows from the ‘Y’
connector to the manifold.