User manual

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8-channel system) are shown below. The 1/32” tubing volume (0.05 to 0.08 ml) is pretty small for a larger
1 ml chamber, but maybe a bit too substantial for a 0.3 ml chamber.
9 cm of 1/32" tubing has a DeadVolume = 0.05 ml
16 cm of 1/32" tubing has a DeadVolume = 0.08 ml
9 cm of 1.0mm tubing has a DeadVolume = 0.07 ml
16 cm of 1.0mm tubing has a DeadVolume = 0.13 ml
10.2.4 Setting up Standard and Pre-Flush Extracellular Slice Automated
Perfusion Systems
To use automated perfusion control for extracellular slice experiments, you have to next decide whether
you want to use a the standard perfusion system with 1 valve/line, or a pre-flush system like AutoMate
Scientific’s AutoPrime system, with 2 valves/line, and you have to choose the number of perfusion
channels you need (see below for further discussion of standard and pre-flush perfusion systems).
WinLTP Slow0 Perfusion Changes offers the following choices for standard versus pre-flush systems, and
number of perfusion channels (see also Fig. 10.2.7.1):
1) 4-channel, PreFlush, 2 valves/line system (low-speed digital Port1)
2) 8-channel, PreFlush, 2 valves/line system (low-speed digital Port1 and Port2)
3) 8-channel, Standard, 1 valve/line system (low-speed digital Port1)
4) 16-channel, Standard, 1 valve/line system (low-speed digital Port1 and Port2)
5) 15-channel, Standard, 1 valve/line system (binary 4-bits on low-speed Port1)
6) 16-channel, Standard, 1 valve/line system (binary 4-bits on low-speed Port1)
For Slow0 Perfusion Change control of extracellular slice perfusion systems, the National Instruments M-
or X-Series boards use 8 or 16 digital outputs for 1 digital output controlling 1 valve. We used this control
first to be able to turn on more than one valve at a time – which is necessary for implementing the pre-
flushing systems. Slow0 and Slow1 Perfusion Change also support digitial control of 15 or 16 channels
using 4-bit binary output, but these outputs do not allow for more than one valve to be on at a time.
The 1 digital output controlling 1 valve method should be able to control the following perfusion systems:
1) AutoMate Scientific’s
ValveLink8.2® 4-channel Pinch Valve Perfusion System
ValveLink8.2® 8-channel Pinch Valve Perfusion System
ValveLink®16 16-channel Pinch Valve Perfusion System
2) BioscienceTools’
8-channel PinchValve System (one PC-16 and one PS-V8)
16-channel PinchValve System (one PC-16 and two PS-V8s)
3) Warner’s
VC-8P 8-channel Complete Perfusion System
4) ALA Scientific’s
VC3-4PG 4-channel perfusion system
VC3-8PG 8-channel perfusion system