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8.3 Sequential Scripting with Perfusion Changes
Although sequential scripting is useful when delivering automated electrical stimulation, a major strength
becomes apparent is when the automated delivery of electrical stimulation is coupled with the automated
change of perfusion solutions during the experiment. Fig. 8.1.1D shows an example of switching from
Ch1 ACSF perfusion fluid to Ch 2 100uM AP5, delivering a LTP induction train in AP5, switching back
from Ch2 AP5 to Ch 2 ACSF wash, and delivering a second LTP induction train. The whole stimulation /
perfusion change sequence is started by exiting the baseline loop by unchecking the Loop event. The
researcher can leave at this time.
Many more examples of Sequential Scripting with perfusion changes are presented in Chapter 10 (see
Figs. 10.3.1.2.3, 10.3.1.3.1, 10.3.2.1, 10.4.1.4 and 10.4.2.4).