User manual

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Fig 8.1.1B shows how this protocol can be produced using the Protocol Builder in the WinLTP Advanced
Mode by checking the check box of the Run statement each time a single T0sweep is to be elicited. The
researcher has to be present each time to check to Run box. Normally the statements located
between the Run and EndRun statements enable more complex stimulations than just the single
T0sweep here.
8.2 Sequential Scripting
Fig 8.1.1C shows how this protocol can be produced using a Sequential Script in the Advanced Mode
Protocol Builder. First, the baseline section contains a Continuous [99999] Loop that contains one
P0sweep (which outputs 1 S0 pulse). At the start of the experiment (eg at the start of the MainProtocol),
the checkbox of this Loop statement is checked. Second, when this Loop checkbox is unchecked
(pointed to by the red arrow), this continuous loop is exited, and the next statement in the Protocol
Builder script is run, a single T0sweep (to output a train of S0 pulses). Third, after this T0sweep, a non-
continuous loop of only 9 loops is run, each loop containing the P0sweep (to output repetitive S0 pulses).
Fourth, then after the 9 P0sweeps are run, the second T0 sweep is run to produce a second S0 pulse
train. And fifth, after this second T0sweep, another non-continuous loop of only 6 loops is run to finish up
the experiment by outputting 6 more repetitive S0 pulses.
For this protocol, the researcher only has to be present to uncheck to Run statement box (when baseline
stability has been reached) to set the rest of the stimulation going automatically. The researcher can
leave if no perfusion changes need to be made.
The following annotated script more clearly explains what is going on in the sequential script of Fig.
8.1.1C.
MainProtocol
‘ Baseline, continuous S0 stimulation
 Loop [99999] ‘ Exit baseline looping by unchecking Loop large check box
 P0sweep [ 2]s ‘ Single S0 pulse
EndLoop
 T0sweep [ 5]s ‘ The first LTP stimulation, S0 train
 Loop [ 9] ‘ Post first LTP S0 stimulation
 P0sweep [ 2]s
EndLoop
 T0sweep [ 5]s ‘ The second LTP stimulation, S0 train
 Loop [ 6] ‘ Post second LTP S0 Stimulation
 P1sweep [ 2]s
EndLoop
EndProtocol