User manual

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A Circular Protocol using Evoked Events
B Circular Protocol with a Run Statement
C Sequential Protocol without Perfusion Changes
D Sequential Protocol with Perfusion Changes
Fig. 8.1.1. Evolution from circular scipting to sequential scripting with perfusion changes. This protocol shows a
series of non-averaging P0sweeps (containing a single S0 pulse) separated by two T0sweeps (containing an LTP
induction train of S0 pulses) A) A circular script for a basic LTP experiment in the Basic Mode. Two single
T0sweeps are evoked by clicking the ‘Single T0’ sweep buttons. B) A Circular script made using the Advanced
Mode Protocol Builder. The two single T0sweeps are evoked by checking the Run statement checkbox (red
arrows). C) A sequential script made using the Advanced Mode Protocol Builder. The entire stimulation sequence
is started by unchecking the Continuous [99999] Loop checkbox (red arrow), and the researcher can leave at this
time if there are no perfusion changes to do. D) A sequential script including Perfuse events to change perfusion
solution between sweeps. The entire stimulation sequence is also started by unchecking the Continuous [99999]
Loop checkbox (red arrow), and the researcher can leave at this time. The bottom tace records Bit1 of Port1 to
indicate switching from Ch 1 to Ch 2 and back to Ch1 solutions.